FastQCFastQC Report
Tue 4 Jul 2023
SRR6880606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13204929
Total Bases660.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2629781.9915139263528034No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2525531.9125661334491082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2159551.6354120495460447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2155231.6321405438832728Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1501971.1374313334058819No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1290270.9771124100705124No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1157710.8767256529739765Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1096020.8300082491924038No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT976360.739390571505534Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC934240.7074933912935086Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA893610.6767245776179486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC877830.6647744944331015No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT864950.6550205608829854TruSeq Adapter, Index 8 (100% over 37bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT831650.6298027047324526No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA752320.5697266528278948No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT739140.5597455313845308Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT673170.5097869136592859Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC654640.4957542747863317Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT648920.491422558955069Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG615010.4657427540882651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG598850.45350489957197043No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC573420.43424693915431123Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC562320.4258409871041336No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC540660.4094380212116248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG538720.4079688728352875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA524760.3973970628694785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC516210.3909222079119093No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT515360.3902785088810398Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA502430.38048671068204915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT491570.37226250894646995No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT488390.36985431727804063No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC439170.33258035692581156Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA437460.33128538593429774Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC422870.3202364813926678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA417980.3165333187327247Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA413410.31307248982557956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC408470.30933146251676175No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT407960.30894524309824006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA401890.30434847472485466Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC386600.29276946509897933Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT378960.28698374675092914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA377240.2856812028296404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT375100.28406059585780435No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG371460.28130404941972803TruSeq Adapter, Index 8 (100% over 49bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC369000.2794411086950941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC369000.2794411086950941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC342520.2593879906510667Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA337650.2556999738506735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA336570.2548820974349805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG336540.2548593786456557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA330620.25037620421889434No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA330550.2503231937104698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC329840.2497855156964494No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG301630.22842228080135835Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC295880.22406784618077083No Hit
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT290200.21976642206860786Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA273100.20681671215346933Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG272780.20657437840067147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGAGCACACGTCTG272610.20644563859449758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC269660.2042116243108918No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG269150.2038254048923701TruSeq Adapter, Index 8 (100% over 38bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC267570.2026288819879304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG266420.20175799506381292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG257670.19513168151074498No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA255470.1934656369602593Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA253660.1920949366709961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAATTCAGATCGGAAGAGCACACGTCTGAA239840.18162914772203623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA238060.18028116622209783Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG236340.17897862230080902No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG230800.17478321920549517No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAGATCGGAAGAGCACACGTCT230750.1747453545566205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG230430.17450302080382257No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT222340.1683765206159003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT219750.1664151318041922No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCT215690.16334052231556867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC215690.16334052231556867No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC211320.16003115200392215No Hit
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA211280.1600008602848224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT211030.15981153704044906No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC206800.15660818774565166No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC198040.14997430126280875TruSeq Adapter, Index 5 (100% over 35bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC193610.14661949337251265Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA188300.14259826766202227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTAGAGGAAGTAAAAGTCGTAACAGATCGGAAGAGCACACGTCTGAAC187860.14226505875192513Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT187720.14215903773507604No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC187250.14180311003565413No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT187010.1416213597210557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC184170.1394706476649742No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT180520.136706528297123No Hit
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT179300.13578263086458095Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAAAGATCGGAAGAGCACACGTCTGAACT174360.13204160355576317Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACGATCTCAACCTATTCTCAAACAGATCGGAAGAGCACACGTCTGAA173980.13175383222431564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG172730.13080721600244877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG172660.13075420549402422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA166760.1262861769268127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCT166660.12621044762906336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT166120.12580150942121687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT165850.12559704031729363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG165190.12509722695214795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT164730.12474887218250094Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT161990.1226738894241688No Hit
TTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTG160930.12187115886802573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC160590.12161367925567794No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA160040.12119716811805652No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT158010.11965986337374476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT157130.1189934455535505No Hit
ACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTG154980.11736526565193951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC154920.1173198280732899Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG153060.11591126313515203No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT149800.11344248802852329No Hit
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC147620.1117915893375875No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG146800.11117060909604284No Hit
TCGTCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA146400.11086769190504545Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG145950.1105269100651734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCACACGTCTGAACTCCA145850.11045118076742405Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG143770.10887601137423761Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTC142980.10827774992201776No Hit
ATGCGTGCATTTATCAGATCAAAACAGATCGGAAGAGCACACGTCTGAAC141050.10681617447545534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA140330.10627092353166004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA138360.10477905636599788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA137810.10436254522837646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC137290.10396875288007985No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC134910.10216639559364538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)