FastQCFastQC Report
Tue 4 Jul 2023
SRR6880607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16227801
Total Bases811.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG4640902.859845274168694No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2950231.8180097229439776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2813211.7335743764666574No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2365411.4576281777179791Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2343441.4440896828843293Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2279021.4043923757753747Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2039761.2569540383197946No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1999271.2320030298621483No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1974291.2166096934513801Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT1552270.9565498122635346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1426790.879225718875897No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1411760.8699638355190578No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1383180.8523520839329988No Hit
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1319930.8133757617560137Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC1049230.6465632651028934No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1032430.6362106609515362No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1011630.6233931510498557Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA1002970.6180566301004061Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC977450.6023305314133443Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA977400.6022997200914653Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC946460.5832336741127155No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT933180.5750501870216427No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA930610.57346648507706Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG921180.5676554697706732Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT889440.5480964426418589Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC862620.5315692495859421No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC849940.5237554983574176Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT798450.49202599908638267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG789340.48641217624002164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA704910.43438417811507546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT670180.41298263393789464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC668050.4116700716258475No Hit
AGGGGCTCGTCCGAAAGCCGCCGTGGCGCAGATCGGAAGAGCACACGTCT657370.40508877327248466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA628460.387273666962024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA591720.3646335076453057Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC589290.3631360774019844Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA540930.33333536688057736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT519150.3199139550700677Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTCGTGTGGAACCTGGCGCTAAACCAGATCGGAAGAGCACACGTCTG480240.29593658438379916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT475620.2930896182421759No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC456050.28103006685871984No Hit
TCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT453360.27937241774162747Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT451130.2779982327858223No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC449110.2767534553819091Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT443920.2735552401708648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT439010.2705295683623431No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC435460.2683419645089313No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC433860.267356002208802No Hit
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA427800.2636216699970625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA426290.2626911680763155TruSeq Adapter, Index 1 (100% over 37bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA425130.2619763454087217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG419660.25860558679515483No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG413830.2550129866640588Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT409950.2526220280862453No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG367470.2264447290178133No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA360050.22187232885096386Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCTTGGTGGTAGTAGCAAATAGATCGGAAGAGCACACGTCTGAACTC347920.21439750216310888Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC343280.21153821149273397Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA342640.21114382657268227Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG340820.21002229445628526No Hit
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC340550.2098559133181384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG326720.2013335016863961Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT318640.1963543920707433Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCAGATCGGAAGAGCACACGTCTGAA315840.19462895804551708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA310140.19111646735130655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG280490.1728453534770361No Hit
AGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAAC278580.1716683609812568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAGGAATTGACGGAAGGGCACCACCAGATCGGAAGAGCACACGTCTGA276790.1705653156579872Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG274760.16931437598969817TruSeq Adapter, Index 1 (100% over 49bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC262590.16181490024433995No Hit
AAATGCAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA259940.16018190018475084Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG259000.1596026473334249No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC257320.15856738691828917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGA253700.1563366472142467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG252300.1554739302016336No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC243530.15006962434405008No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCT242300.1493116658258257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC242270.1492931790326983No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC233190.1436978429794647No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT225840.13916857866324586No Hit
ACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCACACGTCTGAACTCCA225150.13874338242131512Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACCGCCCGTCGCTACTACCGATTAGATCGGAAGAGCACACGTCTGAAC223880.1379607748455875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC223740.1378745031443262Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAGACGAGACCACCGCTCTTGTGTGAGATCGGAAGAGCACACGTCTG219710.1353911106008756Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT219650.13535413701462076No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA219490.13525554078460783No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC218560.13468245019765773Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCAGATCGGAAGAGCACAC217250.13387519356442687No Hit
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA215930.13306177466682023Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC215900.13304328787369282Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTG212460.1309234689284149Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA211070.13006691418017757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC210790.12989437077765498Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC209730.12924117075381933No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA208720.12861878205186272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC208070.12821823486743522No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC206780.127423302762956No Hit
ACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAACT206180.1270535669004075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA204730.12616003856591537Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT204620.12609225365778148Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTC198700.12244419314730319No Hit
AAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAACTCCA198320.1222100271010225Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC191300.11788411750920534No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG190650.11748357032477783Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA187910.11579510988580646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA187260.11539456270137893No Hit
TAGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTG187240.11538223817262733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT184650.1137862116992931Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTAGAGGAAGTAAAAGTCGTAACAGATCGGAAGAGCACACGTCTGAAC184470.11367529094052854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC183630.11315766073296067Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTTTATCCGGTAAAGCGAATGATTAGATCGGAAGAGCACACGTCTGAA183270.1129358192154316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC182240.11230110598472338Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA179400.11055102290199394No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT178830.11019977383257289TruSeq Adapter, Index 1 (100% over 35bp)
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG178570.11003955495880187No Hit
TTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT177190.10918916247494038Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT175540.10817238885293208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGGCGCCTCGCCTCGGCCGGCGAGATCGGAAGAGCACACGTCTGAAC174430.1074883775072174Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA172620.10637300765519618No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC170760.1052268264812959No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC167320.10310700753601797No Hit
ATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAACTC167260.10307003394976312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC165000.10167736220083054No Hit
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC164970.10165887540770312Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCT164770.10153563012018695Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG164490.10136308671766434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)