FastQCFastQC Report
Tue 4 Jul 2023
SRR6880608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15162520
Total Bases758.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG9772696.445294054022682No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA4284032.825407649915713Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC4204322.7728372328610282No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA4133072.72584636326943Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3092262.0394103354851305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT2829411.8660552467531781No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2730361.8007296940086477No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2495841.6460588345472915Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2137551.4097590637967832No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1476180.9735716754207084Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1445630.9534233095817847No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1146530.7561605854435806No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1064060.7017698904931371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1041510.6868976924680067No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT1019290.6722431363651953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1016240.6702315973861864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT973530.6420634564702965No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC838960.5533117186325228Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC805430.5311979802829608Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT791850.5222416854190465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT718620.4739449642935344No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT715050.47159047440662893No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC700320.4618757304194817No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT683260.4506243025565671Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA671880.4431189538414459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG647640.4271321653656516No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT605230.3991618807427789Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT587770.38764664448917463No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG574280.37874970651316536No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG548000.3614174952448537TruSeq Adapter, Index 2 (100% over 49bp)
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT533650.3519533692288617No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC510090.3364150550172399Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC505780.3335725196075586No Hit
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC459290.3029113893996513No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC440770.2906970609107193No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA438010.28887678301496056Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC425810.2808306270989255Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT424490.2799600594096496TruSeq Adapter, Index 2 (100% over 37bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG418630.2760952664860458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC412600.272118354996399Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC408280.26926922437695056Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC390580.2575957030889324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG375230.2474720560962162Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC370210.24416126079306077No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA350100.23089829395113742No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT346210.22833275735168035No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC338000.22291809013277475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA336490.22192221345792124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC332220.21910605888730894No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT330870.21821570556873135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA310700.2049131674682045Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT309100.20385793390544582No Hit
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT303600.20023056853346277No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG300770.19836412416933333Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT298410.19680765466426425No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC290150.19136001139652248No Hit
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT274720.18118360272566827No Hit
ACACGGACAGGATTGACAGATTGAGATCGGAAGAGCACACGTCTGAACTC273020.18006241706523718Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA271510.17906654039038364No Hit
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC271280.17891485056573708Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA269480.17772771280763355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC267650.1765207894202283No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA261970.1727747102724349No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA247580.1632842034173739Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC241080.15899731706866668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC238640.1573880858854597No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC235850.1555480223603992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC234910.15492807264227845No Hit
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC232490.15333203187860592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA229670.15147218272424373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT226880.14963211919918326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACATGGCACCCAAGAAGGCCAAGAGAAGAGATCGGAAGAGCACACGTCTG223220.14721827242437274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA221890.14634110952532955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG219010.1444416891123639Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG217210.14325455135426038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC216070.1425026974407948No Hit
ACGAAGACGAGACCACCGCTCTTGTGTGAGATCGGAAGAGCACACGTCTG214950.1417640339468637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT213830.14102537045293265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA212930.14043180157388085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG210890.13908637878136354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC209000.1378398841353548Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGGCGCCTCGCCTCGGCCGGCGAGATCGGAAGAGCACACGTCTGAAC207330.13673848410422543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC206510.13619767690331158No Hit
ATCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA206240.13601960623959605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGCGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT206130.1359470589321564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC204980.13518860980892358No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT198940.1312051031095095No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG191740.12645655207709536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT187420.12360742145764687No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT185560.1223807124409399No Hit
TCGGGGTCGGGGCACGCCGGCCTCGAGATCGGAAGAGCACACGTCTGAAC185450.12230816513350024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT183880.12127271720004326No Hit
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG183770.12120016989260361No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG179870.11862803808337927TruSeq Adapter, Index 2 (100% over 35bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT179600.11844996741966374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA179100.11812020693130167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA178900.11798830273595683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT178840.11794873147735338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC178640.11781682728200854No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT177720.11721006798342229No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA177380.11698583085133607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG174500.11508641043837041Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA173010.11410372418305137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC170330.11233620796543055Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA170010.1121251612528788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT166600.10987619472224933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT164330.10837908210508543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGGTCGGGCTGGGGCGCGAAGCGGGGAGATCGGAAGAGCACACGTCTG159520.1052067862070421Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT159050.10489681134798173No Hit
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT157500.10387455383405923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT156530.10323481848663679No Hit
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG156090.10294462925687815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)