FastQCFastQC Report
Tue 4 Jul 2023
SRR6880609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18321204
Total Bases916 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG9116894.976141305997139No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG5016002.737811335979884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC3677342.0071497484553964No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT3512201.917013750843012No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3353831.8305729252291496No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3316591.810246750158996No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2832171.5458427295498702Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG2338341.2763025836074966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1460750.7973002210990063Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1418740.7743705053445177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1403450.7660249839475615No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1310440.7152586696813157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1301290.7102644564189122No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1274180.6954673939551135No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1104420.6028097280069585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1026910.5605035564256584Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT994490.5428082128226943No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC961090.524577969875779Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT933970.5097754492554092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC921800.503132872708584No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT837870.4573225646087451No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG793140.4329082302669628No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT748580.40858668458688635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT724560.39547619250350574No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT686530.3747188230642484No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT675820.3688731373767794No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT672970.3673175627540635No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT623260.34018506643995666Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG603650.3294816214043575Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC603230.3292523788283783Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC602020.32859194188329544No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG597280.3260047756686733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT574960.3138221702023513Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG573100.3128069530801578No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT563360.30749070858006927No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG562920.3072505496909482TruSeq Adapter, Index 3 (100% over 49bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG559260.30525286438598687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG558530.30485441895630877No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT534890.2919513368226237No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA528650.28854544712236163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG525680.28692437462079456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTC494020.2696438509172214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA483110.26368900209833374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC479980.26198059909163174No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA470240.25666435459154324No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT456940.2494050063522026No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG456780.2493176758470677TruSeq Adapter, Index 3 (100% over 37bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC437880.23900175992800474Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC437480.2387834336651674Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT412480.22513804223783548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC397100.21674339743174084Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC396360.21633949384549184No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC395490.21586463422382068No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGAGATCGGAAGAGCACACGT389260.21246420268012955No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA385110.2101990677031924Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA375140.20475728560197243Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA353070.1927111340499238No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG349260.1906315763963984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA346780.18927795356680707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC346110.18891225707655457Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC343700.18759684134295979Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT343170.18730755904470034Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT322760.17616746148342652Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG320490.1749284599418248Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC303180.16548039091754013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG302730.16523477387184815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC302450.16508194548786204No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT302000.16483632844217008No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC297030.16212362462641647No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC292760.15979299177062817Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG286510.1563816439137952Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGAAGATCGGAAGAGCACACGT285880.1560377800498264No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA284210.15512626790248066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT283960.15498981398820733No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA283080.15450949620996524No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC277910.15168762926279297No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC267620.14607118615130316Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT267320.14590744145417517No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC264490.1443627831446012Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA264420.14432457604860466No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA259650.14172103536426972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC256250.13986526213015257No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC251950.1375182548046515No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGAGATCGGAAGAGCACACGTCTGAAC248360.13555877659568663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACGGACAGGATTGACAGATTGAGATCGGAAGAGCACACGTCTGAACTC241850.13200551666800936Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC237900.12984954482249092No Hit
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC237400.12957663699394428Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA233490.12744249777470956Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA231500.12635632461709395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT217990.11898235508976374No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA217390.11865486569550777Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA215280.11750319465904095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT215020.11736128258819671No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT214930.11731215917905832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT213870.11673359458253944No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC213830.11671176195625571No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA213380.11646614491056374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA212590.11603495054146006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG210540.11491602844441882No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA205920.11239436010864788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC202090.11030388614198064No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT201120.10977444495460015TruSeq Adapter, Index 3 (100% over 35bp)
ACCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG200080.10920679667122313No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA199410.10884110018097064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA194760.10630305737548688Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT193370.10554437361212723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG192830.10524963315729688No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA191720.10464377777792333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG190750.10411433659054285No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA187820.10251509671525955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC186300.10168545691647776No Hit
TTCGGTCGGGCTGGGGCGCGAAGCGGGGAGATCGGAAGAGCACACGTCTG184930.10093768946625996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT184200.10053924403658188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA183370.10008621704119444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)