FastQCFastQC Report
Tue 4 Jul 2023
SRR6880610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15712095
Total Bases785.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG10829576.892505423369704No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC4291942.731615357468243No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG3589312.284424833225614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2778931.7686565668041085TruSeq Adapter, Index 4 (100% over 49bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2558311.6282424463446792Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2209911.4065024428632846No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2053251.3067958155802903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1980761.2606593837422697No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1975781.2574898509714967No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1849391.1770486367349484No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1831641.1657516072808876No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1573891.0017060105606541No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1553190.9885314466339467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1461150.9299523710873694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT1173720.747016868215219No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1058940.6739648659201717No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT908880.578458824236997No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT842400.5361474711042672No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT786490.5005634194548849No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT785730.500079715658542No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT741500.47192942761611356Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT725700.4618734802710905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC705950.44930354608981166No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT690390.43940034731205485No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT660080.4201094761710644Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG656080.41756366671662815No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT655140.4169654014948357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG618460.39362032879765557No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT578270.36804130830420767No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG576380.3668384133369866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT537980.3423986425743989Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA505780.3219048764661874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC491700.31294362718657187Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTG489390.31147342222663493Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC480050.30552895715052636No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA398930.25389994141455996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC394580.25113137363286053Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT382800.24363396478954585No Hit
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA360300.22931378660834217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC356290.2267616126302699No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT349970.22273923369226065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACAC347050.2208807927905222No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG341830.21755851145248295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC340110.21646381338707538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC337000.21448444653625123Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA336960.21445898844170683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTC331490.2109775940127653No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG329310.2095901278600976Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT326280.20766167719836215No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT323960.20618510771478912Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT313810.19972511622415723No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC310900.1978730398460549No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGAGATCGGAAGAGCACACGTCT310300.19749116842788947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA308940.19662559321338116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC308690.1964664801224789TruSeq Adapter, Index 4 (100% over 37bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA305070.1941625225662141No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA288650.18371197475575343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA277560.17665371804332905Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT271240.17263133910531983No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA270360.17207126102534387No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA269620.17160028627627316No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC267580.17030192345451067Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT256120.1630081793675509No Hit
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG254650.1620725943930456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC253150.16111791584763202Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA251640.16015687277858237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGA250860.1596604399349673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG239750.1525894541752707No Hit
ACGAAGACGAGACCACCGCTCTTGTGTGAGATCGGAAGAGCACACGTCTG239430.1523857894189158Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA237870.1513929237316857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC234620.14932445354995627Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGAGATCGGAAGAGCACACGTC229770.14623765958645235No Hit
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG227870.14502840009559514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT227790.14497748390650642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGCACGCCGGCCAGATCGGAAGAGCACACGTCTGAACTCC218570.13910939311403092Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA213090.1356216341614533No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT213010.13557071797236458Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT212940.13552616630691197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACACGTCT207820.13226753020523363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC207800.13225480115796143No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA203490.1295116914708064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT203050.12923165243081844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG202150.12865884530357027Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTC199400.1269086013036454No Hit
ATTAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCT199230.12680040440183185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA197370.125616603005519Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG195890.12467465350737761Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC194400.12372633948560012No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT192280.12237706047474892No Hit
TCGGCGGCGCGGGGTCTCCCCCCGCCGGGCGTCAGATCGGAAGAGCACAC190060.12096413622753681No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG188790.12015584172575332No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT188640.12006037387121196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT187670.11944301507851116No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA187170.11912478889670665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA184100.11717088014042686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTC183850.1170117670495246No Hit
ACATGGCACCCAAGAAGGCCAAGAGAAGAGATCGGAAGAGCACACGTCTG182930.11642623087500426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTC180910.11514059710051397No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA178040.11331397881695598Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGATCGGAAGAGCACAC175650.11179285766793035No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG174590.11111821816250475No Hit
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA174230.11088909531160548Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC168500.10724222326812562No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGAGATCGGAAGAGCAC167800.10679670661359927No Hit
TTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC167490.10659940638088046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG167030.1063066382936203Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC166440.10593113139909095No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC165610.10540287593729543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC163110.10381174502827281No Hit
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG162250.10326439599556902No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC158700.10100499010475689No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC158340.10077586725385762No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA157970.10054037987932227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)