FastQCFastQC Report
Wed 5 Jul 2023
SRR6880611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47407997
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG45141999.522020092939172No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA42872379.043278078168964No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC37154137.837101829043737No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA18692523.9429044007069107No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC11571412.440813941158493No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG11167112.355532970523939No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9213121.9433683308746414No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA9172121.9347200009314884No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT7721841.6288053680057395Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT7293311.5384134453096594No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA6355931.340687310623986No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG6195461.3068385909659925No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6017961.2693976503584405Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC5714661.2054211022667758No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC5082221.0720174488704932No Hit
ACCGCCCGTCCCCGCCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAG4274240.901586287224917Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG4079090.8604223460442759Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC4026840.8494009987386727No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT2875810.6066086276541065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC2793110.5891643133541372No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG2356990.49717139494419055No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2286710.48234689181236656No Hit
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2051630.4327603210066015Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGT2026690.4274996051826446No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC1953130.41198323565536843No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1887760.3981944227679562No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1734300.36582435659536516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1713920.3615255038089882Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1694620.35745446068940645No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1653330.3487449596320216No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC1641600.3462706935287732No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT1633570.3445768864691752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1548550.3266432032553495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA1545230.3259428994648308Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1525950.3218760750427823No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1484300.31309063742979903No Hit
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1409820.29738020781599356No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC1338540.2823447698075074No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1330480.28064463470160955No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG1266590.2671680054316574No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC1155880.24381540523637812No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG1145410.24160691707772425No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCA1137310.2398983445767599No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1137010.2398350641137612No Hit
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1104370.2329501497395049Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1076990.22717475281649213No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC1059760.22354034489160132No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT984140.2075894495184009Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA954460.2013289023790649No Hit
CCCCCCGGGCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA951320.20066656686634535Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT915740.193161503954702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC899430.18972115611634047No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC897340.18928030222411632No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC882860.18622596521004675No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG878470.1852999611014994No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG836030.17634788493595288Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC784870.16555645664591145No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAGC780730.1646831862565297No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAG767200.16182923737528923No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC757350.15975152884016594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG756330.15953637526597042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC751700.15855974678702414No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG740110.1561150115665085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG703430.14837792029053662No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT691830.14593107572125436Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC677850.1429822061455159No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC636670.13429590792456386No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA630040.13289740969229305Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA619360.13064462520954007Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA618740.13051384558600945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA574140.12110615008687248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC563830.11893141150848452No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT547900.11557121892325467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG535280.11290922077977687No Hit
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT531080.11202329429779537Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCT509240.1074164765914915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCCGCCTCCCCTCCGCGCAGATCGGAAGAGCACACGTCTGAACTCCA507250.10699671618693361Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC503110.10612344579755183No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)