FastQCFastQC Report
Fri 7 Jul 2023
SRR6880612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52866353
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC42933438.121125737574522No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG29269705.536546090100068Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13949522.6386386062984144Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG13351972.5256083013708173No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA11404792.157287074446009No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA10670192.0183329082677597No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9358771.7702696457990965No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT8161831.543860988481653No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT8121501.5362323177466015Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC7811291.4775541637986642No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC5891221.1143609622551418No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG4106080.7766906107557675No Hit
TAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA3768530.7128409254937634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG3745000.7083900794140273No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC2982190.5640998160020609No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA2399500.4538803726445817No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA2358950.446210087539044No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2327730.4403046300545831Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG2293190.4337711738882385Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT2197600.41568972991195363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG2142860.4053353179100514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1891470.35778333338030716No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1765880.3340272025195307Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1724190.3261412793123823Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCA1706290.32275538280463567Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1564240.29588574040656823Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1552090.2935874922183492No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC1530260.28945821172873415No Hit
TTAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTG1491910.28220407032805916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGT1486420.2811656026281972Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC1478520.27967126841528106No Hit
TTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGAACTCCA1433930.27123679214263186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1433560.2711668043377231Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG1422530.26908041112652503No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1362680.2577594107919644TruSeq Adapter, Index 6 (100% over 49bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1279830.24208781717929362No Hit
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1276230.24140685475315463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1202130.22739037814846053Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1201710.22731093253207765Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG1187990.22471570906357016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT1183400.22384748197024296Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1172700.22182351031477432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1169750.22126549943779933Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA1165100.2203859229707031No Hit
TTGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAAC1160540.2195233705642604Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1154830.2184432884939122Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGCCCGTCCCCGCCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAG1129620.21367465994864446Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA1115040.21091676212278157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1071010.20258821333864283No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1050540.19871618532112476No Hit
CCCCCCGGGCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA983280.18599353732609475Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGAGGTGGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG964570.18245442427246683Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC931840.17626334088148657Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCACCCCGAGTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA926300.17521541537015045Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC921220.17425450172437656No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA903800.1709594002067818No Hit
GTGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAAC882730.1669738784515739Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC868790.16433704061258017No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT867620.164115727824085No Hit
CCGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA819680.1550475781826675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA818300.15478654258598093No Hit
AGTGACGAAAAATAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC803120.1519151510224282Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACT799360.1512039235995719Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACA759960.1437511681579397No Hit
AGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT748290.1415437149598725Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTC740750.14011747698957028Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC740140.1400020916895856Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC735120.13905252741758073No Hit
TCCTCCCGGGGCTACGCCTGTCAGATCGGAAGAGCACACGTCTGAACTCC713310.13492703005255535Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC692400.13097177329406476No Hit
AACTGGCTTGTGGCGGCCAAGCGTAGATCGGAAGAGCACACGTCTGAACT673820.12745725055026966Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGACTCTCTTAAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC672420.1271924318289934Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA654270.12375924626387601Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA647500.12247865859027574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC643980.1218128286624954Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGCCGTGCCGGTGGCGGCGGATCTTTCCCGCTCAGATCGGAAGAGCA643680.1217560817936505No Hit
TCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCAGATCGGAAGAGCACACGT637000.1204925181807037No Hit
AACTTCAAAGCAAAATGAAGTTCGTTCTGCTGAGATCGGAAGAGCACACG632970.119730218575887No Hit
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG619250.11713499510737954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAACTCCA605280.11449248258150133Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG605240.11448491633232201No Hit
AGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTGAACTCC594220.11240041468341877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG589430.11149435634419495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCC589410.11149057321960529Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT589030.11141869385240173No Hit
CTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG588010.11122575449832903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGT574760.10871943445767858No Hit
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC574130.10860026603310427Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG569950.10780959299386511No Hit
ACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG559960.10591992226132943Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTCCTTCTGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC559870.10590289820067596TruSeq Adapter, Index 6 (100% over 35bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCTG559650.10586128383018968Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC559440.10582156102199826No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC556430.10525220077125426No Hit
TCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA555180.10501575548440044Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGT555140.10500818923522112No Hit
TTGGGGGGCCCAAGTCCTTCTGATAGATCGGAAGAGCACACGTCTGAACT549860.10400944434355061Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATCTCGCGCCGGGCCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT549780.10399431184519196Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA547190.10350439721083086Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT543240.10275723010437282No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG532410.10070866813907137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)