FastQCFastQC Report
Wed 5 Jul 2023
SRR6880613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39674467
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA33341128.403671812402672No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG32567918.20878324590977No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC24731876.2336993714370506No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA17088784.307248790513052No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACG8851482.2310268213559112No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC8579512.162476436041346No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA7564151.9065536532601686No Hit
ACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6112921.5407692811600973Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT6097651.536920458187882No Hit
ACCCCCCTCCGTCGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT5941261.4975021592602618Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG5666981.428369535499998Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5080671.2805893523408896No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC4766101.201301582703052No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA4726121.191224572720788No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3923010.9887996730995781No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC3562050.8978192448054815No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC3351040.8446339052267545No Hit
TAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGA2947570.7429387772241528Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2305510.581106735472968Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCCGTCCCCGCCCCTTAGATCGGAAGAGCACACGTCTGAACTCCAG2274640.5733259126077233Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT2202530.5551504951534699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC1994870.5028095273466433No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1934730.4876511636564645No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1830160.4612941618094076No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC1580560.3983821635209365No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG1555470.39205819702631417No Hit
ACCCCCCGTCCTTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1439830.3629109875628575Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1379370.3476719674646165No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACACGT1366220.3443574931958128No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1365210.3441029214078667No Hit
CCCCCCGGGCCGCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1322450.33332520888056294Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1307160.32947134488284363Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG1198320.3020380840907075No Hit
TGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC1065910.2686639747422442No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC1054210.2657149748224721No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1021180.25738972120280784No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC951360.23979150116874917No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC939630.23683493971072125No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC938110.23645182177242607No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG930020.23441272695610502No Hit
TGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACACG919130.23166788856924025No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA909380.22921038863609688Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG885590.22321408879922697Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA872140.21982399914786505Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA837350.211055135283859No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA821190.20698198667672085No Hit
TGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG815210.20547472005105955Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT800790.20184014066275927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGT788600.198767635618142Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCT781390.19695034592399188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTG774330.19517086392112087Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCCCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC769330.19391060754514988No Hit
TCGACGCAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA713480.1798335438255541No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC704780.17764069773136462No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC691660.17433378500081678No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCA642740.16200343661831676No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG600370.15132402408833873No Hit
CCGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT591910.14919167030019584Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAGC578780.14588223705689607No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC555530.14002204490863104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCCCGGGGCTACGCCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG554280.13970698081463828Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG522560.13171191436547844No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCAGATCGGAAGAG516130.13009122466597975No Hit
GGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG510370.1286394093208612Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTG505760.12747745294221596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC500000.1260256375970974No Hit
ATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT499680.12594498118903527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTCGCTTCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA483570.12188443514565678Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG474160.1195126326460794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA458260.11550501737049171Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCACCCCGAGTGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA452400.11402799689785373Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA447230.11272489180509974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC441120.11118485851366319No Hit
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA440950.1111420097968802Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC433060.10915332523559801No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT430020.10838708935900765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT428860.10809470987978237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG422720.10654711505009003Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACAC413710.10427613306059032No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACAC407210.10263779977182806No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCAGATCGGAAGAGCACACGTC402030.10133217416632212No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG398210.10036933829508032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)