FastQCFastQC Report
Tue 4 Jul 2023
SRR6880614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46934998
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG719728515.33458039137447No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACG19248734.101146440871267No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC18830834.012108405757256No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT18458433.9327646290727443No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGT12199782.5992927495171085No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA9965962.123353664572437No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC9584772.042137084995721No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC7848371.6721786160510757No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5809261.237724565365913No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC5175861.1027719656022996No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG4754431.01298182648266No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG4620150.9843720457812739TruSeq Adapter, Index 1 (100% over 49bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC4363880.9297709994575902No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3842860.8187621527117143No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCAC3791650.8078513181144698No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA3511830.7482326940761774No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC2563740.5462320462866538No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC2455150.5230957930369997No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2417590.515093235968605No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCA2235180.47622884739443266No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC2193970.46744861904542956No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA1966530.418990110535426No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG1461160.3113156625680479No Hit
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1375740.29311602399556935No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1375580.29308193429559753No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG1301600.2773197092711073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1267640.2700841704520793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA1253900.26715671746699554No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC1233600.2628315867830654No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG1216780.2592479070735233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA1181870.2518099606609123Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1165880.24840312126997427No Hit
ACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGT1155710.24623629471551273No Hit
TTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1136640.24217322860011625No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACA1130890.24094813000737741No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG1087540.23171195192125074No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1070990.22818579858041116No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC1034090.22032386152440017No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC994810.21195484018130778No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC991990.21135400921930367No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA954650.2033983254883701No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG882980.1881282705072236No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG852500.1816341826625837Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT840150.1790028839460055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCAGATCGGAAGAGCA827140.17623096521704337No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCAC821820.17509748269297892No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA795470.1694833352288627No Hit
TAACCCCGCGGGGGCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGT787320.16774689113654592No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC759690.1618600260726548No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC759510.16182167516018642No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA738710.1573900141638442No Hit
ACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGTC733630.15630766618973754No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG715210.15238308948047682No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC706230.15046980506955598No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA698950.1489187237208362Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC697050.14851390853367033No Hit
AACCCCGCGGGGGCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGTC688850.14676681141011233No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA684170.14576968768593535No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCAC669000.14253755800735307No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACACG632700.13480345732623658No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG631260.13449665002648983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCTCCGTCGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG609510.12986258143656465Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCA609200.1297965326428692No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCAC598330.12748056365103072No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC569040.12124001794993151No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGCA566100.12061361971294852No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT561100.11954831658882782No Hit
ATAGCTTTGGAGACCTATCCTCTGCCTCTGAGATCGGAAGAGCACACGTC554100.1180568922150588No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC530290.11298391873799589No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCAGATCGGAAGAGC529120.11273463780695164No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC514270.1095706875283131No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT497550.10600831388125338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)