FastQCFastQC Report
Tue 4 Jul 2023
SRR6880615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46153365
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG854038418.504358241267997No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT23381135.06596431267796No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACG22370064.846896862233122No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC14155373.0670288071086476No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC12238212.651639810011686No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA11696692.5343092535073013No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC10365982.245985747734754No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC8116661.7586280003635706No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGT7663211.660379476122705No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC7344861.591402923708813No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA3899100.8448138071839398No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3886530.8420902787911564No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3746160.8116764617271135No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC3369840.730139611705452No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG3105620.6728913482256386No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2744750.5947020331020284TruSeq Adapter, Index 2 (100% over 49bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA2677720.5801787150297708No Hit
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2437070.5280373381225832No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG2167480.46962556251315585No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC2030290.4399007526320129No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1643180.35602604490485146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA1621580.35134599611534284No Hit
ACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGT1563190.3386946975588887No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG1516250.32852425819872505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1401320.3036224985978812No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1346080.2916537071565638No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG1243720.26947547594850346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC1222550.26488859479693405No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC1175810.2547614892218585No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC1164240.2522546297545152No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA1133880.24567656117815026Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC1112620.24107017982329132No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1109960.24049384048162037No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1090370.23624929623224655No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1044530.22631719269006711No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG1004190.2175767682378089No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC995680.21573291568231265No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC943860.20450513196600076No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC905530.19620021205387733No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG879850.19063615404857262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA875090.1896048099634772No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC870530.1886167996634698No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA870290.18856479912136417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC869520.1883979640487752No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA840460.18210156507548259No Hit
ACCCCCCTCCGTCGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG835410.1810073870020095Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC797820.17286280209471183No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC729690.15810114820447005No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC723070.1566667999180558No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGT716210.15518045108953593No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC678620.1470358661822383No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT665640.1442235035300243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG654300.14176647791553226No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT618120.1339273961931053No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT610390.13225254539945247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG600910.13019852398627924No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA594340.12877500914613701No Hit
ACGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA577010.1250201366682581No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT565220.12246561003731798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC557310.12075175883708587No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGCA554250.12008875192523881No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG550740.11932824399694367No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGT550670.11931307717216286No Hit
ACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGTC545860.11827089964079542No Hit
TTGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT510530.11061598650499264No Hit
AAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACGTCT506770.10980131134533744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCTG479000.10378441528586269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTACTTCTGATCGAGGCCCAGATCGGAAGAGCACACG465390.10083555121062136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)