FastQCFastQC Report
Tue 4 Jul 2023
SRR6880616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38232580
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG622486116.28156143268385No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACG22269895.824846243701052No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT19362345.064356106755024No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC16775644.38778654226317No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC13182103.4478708996358605No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC10441032.7309247767218428No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA9551132.4981651774481346No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACGT7255891.8978290243556672No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6640651.73690867840988No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC5723461.4970111878403185No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA4483321.17264385505765No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3352330.8768254718881122No Hit
AACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCTG3315570.8672106355364979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG3133290.8195340204610833No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACAC2837370.7421340647165323No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2473450.6469482310636635No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA2419740.632900003086373No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG2352880.6154123001900473No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG1882020.49225555795606785No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC1794310.46931439102461825No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA1431700.3744711970785126No Hit
TAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT1410780.3689994240514242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC1336320.34952388774181603No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1296190.3390276042056278No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1288690.3370659264951515Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1248380.32652256269391183No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1248000.32642317102324775No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1230990.32197408597588756No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1221160.3194029803900234Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACA1162590.3040835852563442No Hit
ACCCCGCGGGGGTTCCGGAGCGGGAGGAACCAGATCGGAAGAGCACACGT1139060.2979291483860101No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1077020.2817021503649505No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCAGATCGGAAGAGCAC1002410.26218738050113277No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC987520.2582927963532673No Hit
TTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG971770.25417327316126714No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCAGATCGGAAGAGCACACGTC948550.2480999189696327No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC910360.23811105606788766No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG882880.23092346893670268TruSeq Adapter, Index 3 (100% over 49bp)
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAACAGATCGGAAGAGCACACG856200.2239451274279685No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGC823130.2152954365099086No Hit
ATGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG815910.21340699476729008No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG813220.21270340636179927No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGT765220.20014866901475128No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA751690.19660980242505216No Hit
CCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACAC731050.1912112653658215No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA682740.17857544534007383No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG623320.16303372673254068No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCAGATCGGAAGAGCA587320.15361767372225468No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC582430.15233865985502418No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT576070.1506751571565403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCAGATCGGAAGAGCACAC559330.14629669250675734No Hit
TCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGT559170.14625484338226719No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT546760.14300892066399915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCTCCGTCGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG546130.14284413973631913Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC542380.141863300881081No Hit
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC526000.13757899676140087Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG519010.135750713135237No Hit
ATCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC514940.13468617603101857No Hit
GTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC510310.13347516699108455No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCAGATCGGAAGAGCACACGT484990.12685254304051677No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG484680.12677146036181708No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCAC476570.12465023286422208No Hit
CTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC452450.11834147734733047No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC447280.1169892275122422No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACTCAGATCGGAAGAGCA435790.11398393725979257No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG424490.11102834284267502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG420210.10990887876256325No Hit
AAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGTCT401800.10509361387591422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCAC386220.10101855537868488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)