FastQCFastQC Report
Tue 4 Jul 2023
SRR6880617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32815224
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG7650492.3313843598934443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCTGAACTCC4499011.371013039557493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA3471351.0578474186249651Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA2154460.6565428290235045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1830160.5577167475681409Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCTGAACTCC1663360.5068866816207014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG1404800.42809398467004217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1251740.38145099969453206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATTGATCATCGACACTTCGAACGAGATCGGAAGAGCACACGTCTGAAC1167050.35564285649855687Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1126520.3432918818411844No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1108800.33789194917578497No Hit
TTCGGTCACGCGTCTCCCGACGAAGCCGAGATCGGAAGAGCACACGTCTG1015730.30953011321818186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCAGATCGGAAGAGCACACGTCTGAA973660.2967098441869542Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT964410.29389103057775867No Hit
CCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCAG909950.2772950749932409Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG890570.2713892795612183TruSeq Adapter, Index 4 (100% over 49bp)
CCCCGCCCCTTGCCTCTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA850430.25915715218034163Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC813550.24791846613632745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT779210.23745381107256802No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC770670.23485136045391614No Hit
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCAC763800.23275781996795145No Hit
ATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAACT741880.226077993555674Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC712160.2170212216134804Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA700680.2135228453720139Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAAC697120.21243798305323167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC679460.20705633458421613TruSeq Adapter, Index 4 (100% over 38bp)
TCGCGTCTCTCGGGGTCGGGGGTAGATCGGAAGAGCACACGTCTGAACTC663830.2022933014261917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGAGATCGGAAGAGCACA660150.2011718707146415No Hit
ATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACT646520.19701831076941603Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT644560.196421027020873Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG639620.19491562818525937No Hit
TTCGGTCACGCGTCTCCCGACGAAGCCGAGAGATCGGAAGAGCACACGTC635630.1936997291257253No Hit
TTGGCGTACGGAAGACCCACTCCCCGGCGCCAGATCGGAAGAGCACACGT631370.19240155118246335No Hit
TCGCCTCGGCCGGCGCCTAGCAGCCGACTTAGATCGGAAGAGCACACGTC614100.1871387499899437No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCAGATCGGAAGAGCACACGTCTGA599750.1827657796881106Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCACGCGTCTCCCGACGAAGCCGAGATCGGAAGAGCACACGTCTGA572370.17442209140489182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCAACCTATTCTCAAACTTTAAATGAGATCGGAAGAGCACACGTCTG568330.17319095551503777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC565700.17238949824020705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCCA561920.17123759386801687Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC555770.16936346373865985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA554910.16910139025715626Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG552270.16829688561626152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC545470.1662246766927448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGA544390.16589556115783333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT542840.16542321941791407No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA540110.1645912884824434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCT529060.16122394898172873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT524710.1598983447438908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT518810.15810039876613366Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA515100.15696982595639147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTATGGTTCCTTTGGTCGCTCGCTCCAGATCGGAAGAGCACACGTCTGAA504120.15362381801812477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG497740.15167959846929582Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCC485080.14782163303227794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA484230.14756260691683837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCCCGTGCCTTGGAAAGCGTCGCGAGATCGGAAGAGCACACGTCTG483730.1474102386136386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCACGCGTCTCCCGACGAAGCCGAGAGATCGGAAGAGCACACGTCT478220.14573113991237727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGGCATGCTAACTAGTTACGCGACCCCAGATCGGAAGAGCACACGTCT474640.144640182861467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA469880.14318963661500528Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCT467470.14245522139358244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGATCGGAAGAGCACACGTCT451090.13746363578075835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGGTCGGGCTGGGGCGCGAAGCGGGGAGATCGGAAGAGCACACGTCTG440890.13435532239548326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA433200.13201189789227097Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG430580.13121348798350424Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCGTCTCTCGGGGTCGGGGGTGCAGATCGGAAGAGCACACGTCTGAAC424490.12935764205053119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGAAGGTGGCTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA416300.12686184924411914Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC414000.12616095504940025TruSeq Adapter, Index 4 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGAGATCGGAAGAGCACACGTCTGAAC411060.12526502942658566Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTC410990.1252436978641377No Hit
TTCGGTCACGCGTCTCCCGACGAAGCCGAGCAGATCGGAAGAGCACACGT395350.12047761734004923No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA392420.11958473908329866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT388920.11851816096090034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTCT381550.1162722521717359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGATCGGAAGAGCACACGTCTG379200.11555612114669703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC372700.11357533320510017Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGAGTGCCGTTCCGAAGGGACGGGCGATGGAGATCGGAAGAGCACACGT362860.11057672499812893No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA360150.10975088879478623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCACA359670.10960461522371445No Hit
TCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGAGATCGGAAGAGCA359490.10954976263456255No Hit
CCCCCCGCCTCCCCTCCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT350810.10690464889101473Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCT350740.10688331732856676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGCCATGCATGTCTAAGTACGCACGAGATCGGAAGAGCACACGTCTGA350330.10675837531994296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTCTTAGCGGTGGATCACTCGGAGATCGGAAGAGCACACGTCTGAA347980.10604224429490411Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA347220.10581064447404047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCTGAA345970.10542972371604108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC343630.10471664005706618No Hit
GTCTACGGCCATACCACCCTGAACGCAGATCGGAAGAGCACACGTCTGAA343100.10455512965567444Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGAA335180.10214161573299028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA333680.101684510823391Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT333440.10161137403785511No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGGAGATCGGAAGAGCACACGTCTGAA333100.10150776359167929Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTG332020.10117864805676781Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATCTCAACCTATTCTCAAACTTTAGATCGGAAGAGCACACGTCT330700.10077639573632044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)