FastQCFastQC Report
Tue 4 Jul 2023
SRR6880619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15364755
Total Bases768.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC8018385.218683929551757No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2963621.9288429916389813No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2704521.7602102994808573No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2563811.66863057692752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2207341.4366255758715318Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1993981.2977623138149617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1947021.26719885868665Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1899511.2362774414561117No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1756001.1428753663823472No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1529080.995186711405421Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1376590.8959400914625713No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1146490.7461817646945884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1114890.7256152148211931No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1064200.6926241257995979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1014170.6600625913006748Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG884530.5756876696048847No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA869870.5661463524800754Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA823960.5362662795469242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT727530.4735057604237751Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA700640.456004667825813No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC661780.43071301820302377No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA650420.4233194736915753No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA646020.4204557768737608Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG643750.41897836965184276Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT581480.37845055127790844No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG576830.3754241444136272No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC569820.3708617547106999No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC525680.3421336689065332No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC504550.32838141577916474No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT487130.31704377974136266No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT477240.31060697030313855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC471970.30717704252361977No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA464250.30215255628872706Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC449120.2923053442765602No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC446620.2906782438118929No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC443830.2888623996933241No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC412690.2685952363054276No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT405730.2640653886117937Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC395160.2571860078471801No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT392310.25533111331745934No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA373600.2431538934398889Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT367520.2391967851098179No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC362790.23611831103066727No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG358090.23305936215709264No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG349210.22727990130659423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA343820.22377187270477142No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG342110.22265893598693892No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA341780.22244415872560283No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT330760.21527189987734915No Hit
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT327480.2131371440677056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT321970.20955101464357875No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT320060.2083079098885729No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG318390.20722100677817512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG302800.1970744082805095No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA299130.19468582479837784No Hit
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC279220.18172759669776706Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC275600.17937155522492876No Hit
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG275430.17926091239333136Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG274030.17834973613311764No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC271500.1767031104628743No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC268610.17482218232571883No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC267600.1741648337379932No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC256330.1668298648432728No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC255120.1660423482183738No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA253190.16478622665965062No Hit
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGC252890.16459097460389052No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG249380.16230652555149758No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA249190.16218286591618286No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAG248080.16146043330987056No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG244360.15903930781844552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC240970.1568329595883566No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAG236430.1538781451445207No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT233470.15195165819435455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG217070.14127787914613674No Hit
TTGGCGCACTGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA216800.14110215229595266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT215830.1404708373156617No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT213920.13922773256065585No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC213140.13872007721567964No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC211740.1378089009554659No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC209830.13656579620046008No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAAAGATCGGAAGAGCACAC205040.13344827171015744No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC204980.13340922129900543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG204120.13284949873915985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG204070.1328169567298665No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA202200.13159988558229532No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA199720.12998580192134532Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC196300.12775992848568038No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA194650.12668604217899992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC189130.1230934043530144Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC188530.12270290024149424Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA188000.12235795494298478No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC186480.12136867786046701No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC183740.1195853757511916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT183450.11939663209729019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA179040.11652642687761698No Hit
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT178000.11584955308431538Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC177950.11581701107502203No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACA177740.11568033463598996No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT177560.11556318340253391Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC174220.11338937718173835No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC173000.11259535215498066No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC172320.11215278082859115Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT171580.11167115909104962No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG168040.10936718483308065No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG164520.107076227378829TruSeq Adapter, Index 5 (100% over 49bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT161060.1048243203357294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT160130.10421903896287316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC159340.10370487521603826Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC159180.10360074078629956Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA155400.10114056488372253No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA154240.10038559026811686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)