FastQCFastQC Report
Tue 4 Jul 2023
SRR6880620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35726539
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG17851144.996604904829992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC12259503.4314826857423832No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC8481322.3739551149916873No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA8326752.3306903587834245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG7977482.23292830016364No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT5975151.672468189543913Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA5785751.619454378158489Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG5031851.4084347772953882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC3983551.1150114484921139Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG3457220.9676895934420069No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA3340890.9351283649390163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA3213600.8994993889556444No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT3007090.841696420691632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2698450.7553068602587002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2285100.6396085554215033No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT2244780.6283228274644795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG2224010.6225092220659829Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2124490.5946531792514242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1878300.5257436215693886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1816970.508577111261743No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1617800.45282863811689117No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1548810.43351806342058496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1493510.41803937403508357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1415750.3962740415465378No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC1400480.3919999079675756Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1346190.37680392158893417No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1312380.3673403684583049No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC1308880.3663607045731466No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT1224730.34280678573426887No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1113820.31176263673343785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT1081540.3027273366726063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1017400.28477429621716227No Hit
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1000990.2801810721156057Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT995490.2786416002960712No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA983170.27519318342031396Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT964100.2698554147660371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG891800.24961835793833823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA876670.2453834109147824Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG873950.2446220721240308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC792350.2217819084014827No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC783430.21928516501416498No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG781220.21866657724667926No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC780030.21833349152572545No Hit
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC741180.20745922240046816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC698840.19560808842972446Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC696260.19488593619437922No Hit
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG687530.19244237456082716Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG684890.1917034280874506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT684710.19165304537335676Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG661900.18526843588179642No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT651450.1823434394246809Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC625040.17495117565124346Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC621020.17382596170314735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC617190.17275392950881696Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA616640.17259998232686352No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG611990.17129842887943889No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG606340.1697169714648262Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA605680.16953223484648205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC600120.16797596878891627No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT594910.16651766911986632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT586520.16416927483515825No Hit
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC585300.1638277919951888Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG580430.1624646596749828TruSeq Adapter, Index 1 (100% over 49bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT560490.15688337456925228No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT556080.1556489980739528No Hit
CCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACTC539400.1509801999012555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC533200.14924479530468931Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA533020.1491944125905955Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG528600.1479572370556241No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT521820.14605948815808886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA517190.14476353279000803Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG517100.14473834143296108No Hit
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC510350.14284898965444146Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG508540.14234236347383103No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC499870.1399155960783103No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG496520.1389779177882302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA486860.13627404546519326No Hit
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTG486810.13626005026683385Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT483150.13523560174692545Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT477610.13368493376870344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA472500.1322546244963723Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC468830.13122737693679198TruSeq Adapter, Index 1 (100% over 38bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA466850.13067316708175958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT460280.12883419801733384TruSeq Adapter, Index 1 (100% over 35bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT459750.12868584891472415No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC459690.12866905467669287No Hit
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC459020.12848151901867683No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA457390.12802527555216026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC451550.1263906363837818Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT451180.12628707191592223Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA448790.12561810143434268No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA446330.12492953767505997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA432000.12091851382525466Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG430990.12063581081839468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTGAACTCCA424020.11868488016709372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC415730.11636447627910444No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG407800.1141448378193029No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC397590.1112870183143125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG394140.11032134962751361Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC392000.10972235513773108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA390350.10926051359187075Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA384780.10770144849463308Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC381440.10676656924422487Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT377180.1055741783440036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGT363110.10163592952566719Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)