FastQCFastQC Report
Tue 4 Jul 2023
SRR6880621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21064418
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG7051383.3475313678260656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA5078062.4107288414044956No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT4260932.0228092701160794No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3799431.803719428659268Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2419841.1487808492976166No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC2145651.0186134741534278No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1902800.9033242693911601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1747150.8294318884101142Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA1688690.8016789260448591Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1545510.7337064807582151No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1540700.7314230091712005No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1488860.7068127873269511Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1457260.691811186048435No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1389550.6596669321696901No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1377980.6541742572711954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1355190.6433550644503921No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT1280630.6079588811805766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1266790.6013885596079607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC1263480.5998171893474579Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA1150420.5461437386971717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1128170.5355809023539126No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1066760.5064274740465177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1004050.47665689125614574Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC896600.4256466995670139Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG804830.3820803404110192No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA752020.3570096263756255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT746280.3542846519661735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG714210.3390599256053502No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT689800.32747166335191413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT688390.326802288104993Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG682540.3240250929315968No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA663200.3148437331617707Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT636310.3020781300485017Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT630540.29933891361251946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC629500.29884519002613796No Hit
TCCGTCCCCGCTCGGCGGGGTCCCAGATCGGAAGAGCACACGTCTGAACT624660.296547476412593Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG611900.2904898677950656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT607870.2885766888978371No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG595660.2827801841000307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT570760.270959302079934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA547880.26009738317954No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA544620.25854974962992094Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT540130.25641819299256213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA513290.24367632659017688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC494910.23495071166931836No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC464010.22028142434317435No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC459290.2180406788357504No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA450500.213867765062391Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG436580.20725946475236107No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT435390.20669453103332835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG433760.2059207142585188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA421450.20007673603894494No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT417480.1981920411947769Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG409120.19422326313501753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT405020.19227685284255183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA402950.19129415301196548Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC383330.18197986766119054No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG382260.18147190204827876Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTGAACTC381780.181244029623795Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC363100.17237599443763413No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT362510.17209590124920612No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTCTGAAC357140.16954657850029373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA355680.16885346654248887Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT333150.15815770461828094No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC329310.1563347252224106No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA328940.156159073561871Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG321810.15277421858985138Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA307100.14579087824785852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT305350.14496009336692806Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTC304080.14435718091048136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGTCTGAACTCC297570.14126666115341996Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC293870.13951014454802407No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACG292310.13876955916845177No Hit
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA275930.1309934126829424Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC275610.13084149773328654No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA272910.1295597153455652No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGT272340.1292891168414907No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG271990.12912295986530462No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT270340.12833964840614157No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT264380.1255102324688012No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA263920.12529185472867088No Hit
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT261750.12426168147631708Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT259630.12325524493484699Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT244730.116181705091496No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC244040.11585413848130056Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA235570.11183313965759699Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG232770.1105038838481082TruSeq Adapter, Index 8 (100% over 49bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG229250.10883281940189377Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACT228800.1086191890039402Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT228370.10841505329034014Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT228350.10840555860598665Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC227380.10794506641484232Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCA225460.10703357671690715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG222820.10578027838224631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA221130.1049779775543763Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG220260.10456495878499943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)