FastQCFastQC Report
Tue 4 Jul 2023
SRR6880622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20352876
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG4395542.159665297425288No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA3928301.9300957761448554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC3455651.697868153866805No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG3317121.6298040630719708No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT3288831.615904307577956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC3253351.598471881811691Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2885361.4176669675578035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA2878321.414207996943528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2509811.2331475905419953No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2358291.1587011093665582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2145601.0541999076690685No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2101291.0324290287033637Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC2045521.0050274958683971TruSeq Adapter, Index 1 (100% over 38bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2041271.0029393388924495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA1783340.8762103203498119TruSeq Adapter, Index 1 (100% over 37bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1712900.8416009609649271Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1706620.838515401951056No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG1612850.7924432890958507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1428760.7019941555188565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1345540.6611055852745332No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1328440.6527038242654257Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1243360.6109013782622171Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA1235330.6069559899053086No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1233740.6061747735307776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1063410.5224863552453226No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC1045940.5139028017465443No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA911670.4479317812381896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG859350.4222253405366396Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT853880.41953775967583157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG845210.4152779194448981Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT800520.3933203346789908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA757530.37219801270346264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT749850.3684245902151617No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT741780.3644595486161268Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACT725380.3564017193442342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC709070.34838810986712637Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA623320.3062564720582978No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC591310.2905289650465123No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC590240.29000324081962664No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT580090.28501623063001025No Hit
TTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAAC575750.28288385385927767Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC553870.2721335304160454No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA529870.260341585140105No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC520520.2557476397930199No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA519680.25533492170836203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG508450.24981727398132825No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT502950.24711495318892523No Hit
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC502680.24698229380457093Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC489890.24069816963460103No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA473250.23252242090994907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA470310.23107790761364636Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC461440.22671980117208004No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT451490.22183105719309645No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA451350.22176227084565345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC443140.2177284428991755Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG398050.19557432571200256Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC395060.19410524586304165No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT392510.192852351677473No Hit
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT390590.19190899605539777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC385850.18958008686339956No Hit
ATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA371660.18260809921899981TruSeq Adapter, Index 1 (100% over 37bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC369630.18161069718107653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG341000.16754388912898602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA338340.1662369485275693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC319700.15707853769658892No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCAGATCGGAAGAGCACACGTCTGAA315910.15521639300509665Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT315030.1547840216783122No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG311670.15313314933968056TruSeq Adapter, Index 1 (100% over 49bp)
AAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG304910.14981175142029068TruSeq Adapter, Index 1 (100% over 39bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT303760.14924672070915185No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA303000.14887330910874708Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC300470.1476302415442417No Hit
TCGGGGTCGGGGAGCTCGGAAGAGCACACGTCTGAACTCCAGTCACATCA297020.14593514941082528TruSeq Adapter, Index 1 (97% over 37bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA295490.14518341289948408No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG292870.14389612554019393Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA291220.14308542930247303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA284630.1398475576621211No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC281040.13808367918126166Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA276240.13572529012607357TruSeq Adapter, Index 1 (100% over 37bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC275260.1352437856939727No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC272090.13368626625544222Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA260430.12795734617554788No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA259230.12736774891175084Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC256710.12612959465777712No Hit
TGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCC256080.12582005609428368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG255720.12564317691514457Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC253150.12438045610851263No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG250390.12302438240177947No Hit
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC244770.12026310188299677TruSeq Adapter, Index 1 (100% over 36bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG244510.12013535580917409No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG237740.11680904457925258No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA235040.1154824507357093Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC231410.1136989190127233No Hit
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGC230160.11308475519626808No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG228030.11203822005302838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT227580.1118171210791045No Hit
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC224140.1101269422562197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT216700.1064714392206782No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC213640.1049679661979958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGAGGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC212070.10419657644452805TruSeq Adapter, Index 1 (100% over 38bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG205900.10116506384650503No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)