FastQCFastQC Report
Tue 4 Jul 2023
SRR6880623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30948664
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG11390983.680604758900093Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6045571.9534187323885774No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT5946011.9212493308273337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG5624311.8173030021586716No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC5561171.7969014752947008Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC4471931.444950903211848No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC3352561.0832648543407237No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT3305781.0681495007345065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3250291.050219809165268Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA3242641.047747973870536Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT3232181.0443681833891116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA3148261.0172523117637646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC3144661.016089095154479Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC2635890.8516975078471885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2622520.8473774506065916No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2613150.8443498562652009No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2606920.8423368452996873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2516670.8131756511363463No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT2332170.7535607999104582No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT2210980.7144024052217569Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2026530.6548037097821089Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1784650.5766484782671071TruSeq Adapter, Index 1 (100% over 49bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1744990.5638337086214772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1698200.5487151238580121Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1633630.5278515415075753Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1490000.48144242995432696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1477750.47748426232550784Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1467700.47423694929125215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA1441780.4658617897043957No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1390040.4491437821031628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG1389010.44881097290661726No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG1387890.4484490832948395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA1383700.44709522840792093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1360990.43975727029767747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1343520.43411243858539417No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1286310.4156269879694969Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1268600.40990460848326116No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1227110.39649853706124444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1041270.33645071076412214Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT981960.31728671712614154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC957880.30950609047291994No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG927860.2998061564143771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC906220.2928139321296713No Hit
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT889720.2874825226704455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAAC873390.28220604288443596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT826800.26715208126593126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT824010.26625058839373483Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC789490.255096633573585No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT787970.25460549767188656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG769930.2487764899964664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA763380.24666008199901618TruSeq Adapter, Index 1 (100% over 37bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA751840.24293132653480615No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC716380.23147364293334277No Hit
CCCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA697420.22534736879110517Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA688290.2223973222236669Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA686330.22176401540305585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGA673940.2177606115727645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC664210.21461669557044533Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG660560.2134373231749196No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA656780.21221594573516972Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG647240.2091334217205628No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAA647010.20905910510385844Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC639870.2067520588287753TruSeq Adapter, Index 1 (100% over 38bp)
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC627350.20270664995425974Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG616250.19912006540896238Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT615600.1989100401878414Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT562300.18168797205591816No Hit
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA553100.1787153073877438Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT541620.17500593886702187No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT520920.16831744336362953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA518610.16757104603933792Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG504750.16309266209358828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG504040.16286324992897916Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAAC501540.16205546061697526Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC500340.1616677217472134Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT499100.16126705824845944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC494970.159932590305029Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC494890.15990674104704486Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC471760.15243307433238476Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA464900.15021650046024604No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC464370.1500452491261012No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG461230.1490306657502243No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCAGATCGGAAGAGCACACGTCTGAA456700.14756695151687324Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA441240.1425715824114411Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT439450.1419932052640463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC436110.14091399874320906No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA420850.13598325278273724Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC412030.1331333720899875No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC404740.1307778584561841Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC397940.12858067152753347No Hit
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG390680.12623485136547413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC389320.125795413979744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT387750.12528812229180555Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG385140.12444479025007349No Hit
TTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACACGTCTGAACTCC384100.12410874989627985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC377840.12208604545902207Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG372450.12034445170234166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCAGATCGGAAGAGCACACGTCT363550.11746872175160776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT360100.11635397250104237Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT355850.11498073067063574No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG354210.11445082088196118No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT349590.11295802623337795No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA341650.11039248737845357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGAAC337190.10895139124583858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG332350.10738751113779904No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA332010.1072776517913665Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC331550.10712901855795778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT327670.10587532954572773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA317480.10258278030999982Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT316230.10217888565399785Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA314010.10146156874493839Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)