FastQCFastQC Report
Tue 4 Jul 2023
SRR6880624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34003216
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC8949662.6320039845642835Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6720651.9764748134411758No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC5893781.7333007560226068No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT5567831.6374421760577No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG5486711.613585609078859Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG5047241.4843419516553964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG4606151.354621868707948TruSeq Adapter, Index 5 (100% over 49bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA4055201.1925930770783564No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA3203240.9420403058346011Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT3087520.9080082307508796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3013520.8862455833589387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC2949310.8673620753989858No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC2878820.846631683309014Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2794100.821716392943538No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2788680.8201224260669931Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC2773730.8157257831141619Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2627870.7728298405656688Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT2585650.7604133679590778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2307780.67869462700234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2221250.6532470340452503Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT2205990.6487592232452366Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG2147240.6314814457550133No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2100840.6178356776606071Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2047750.6022224486060377Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1809020.532014383580659Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1723560.5068814667412636No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1666460.4900889374699146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1655630.4869039446151211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1615250.47502859729503233No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG1455460.4280359834199212TruSeq Adapter, Index 5 (100% over 37bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1452410.4271390094395778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1419110.41734581811320437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1397210.4109052508445084Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1394970.41024648962615773No Hit
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT1340470.39421859391182296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1320200.38825739306540885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGA1261380.37095902928711216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1200120.3529430863245406No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGT1181110.3473524386634488No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1147690.3375239565575209Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG1045800.30755914381745536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1012440.2977483071013048Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG1006040.2958661321917315No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA999260.2938722031469024Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC989100.2908842504779548No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT975580.2869081559814813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG931610.27397702617305375No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC909660.2675217544128767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG902260.2653454896736826No Hit
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC877310.25800794842464314No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG873860.2569933385124513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC872710.25665513520838734No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC849170.24973226061911322No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA838520.24660020393365145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTC788660.23193688502875726No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACG772750.22725791583948998No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA761510.22395234615455198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT750650.22075853060486983TruSeq Adapter, Index 5 (100% over 38bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA740030.2176352966142967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG729990.21468263472490365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG712270.20947136294402272Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA708950.20849498470968159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT702550.20661280980010832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA659050.19381990221160256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA626720.1843119780199614Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC616770.18138578421523424No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTC579470.17041623357037758No Hit
TTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTG562290.16536377029749186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG554860.16317868286340914No Hit
TTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAAC540990.15909965692656836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA539920.15878498080887407Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGT524700.15430893360204517No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA524040.15411483431449544No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT516890.15201209203270655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGAA512420.15069751049430147Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT510110.1500181629878774No Hit
TCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACAC500690.14724783679284925No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC487370.1433305602622999No Hit
TCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTGAACTC486190.1429835342633473Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC453960.13350501905466824No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG452680.1331285840727536Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA438080.12883487256028958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG432590.12722031939567127Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC429710.12637334068636333No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA426010.12528520831676626Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA415220.12211197905515761Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC396000.11645957252984541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGGTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCT388720.11431859857020583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC384810.11316870733638841Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG382600.1125187688129264No Hit
TCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACACGTCTG379040.11147180901947627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCA377940.11114831020689338Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC373940.10997195088841008Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC368800.10846032916415906Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT362430.10658697694947443No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC360450.1060046790868252No Hit
TCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACA358770.10551060817306221No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT357260.10506653253033477No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT356880.10495477839507887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG352950.10379900536466903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG349480.10277851365588478Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC347290.10213445692901518No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)