FastQCFastQC Report
Tue 4 Jul 2023
SRR6880625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42314896
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC17918984.234674238594371No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG9375892.215742182138413No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC8974512.1208866967320446No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC5258421.2426876814254724No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG4562451.078213686263107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG3970790.9383905847245849Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3509960.8294856733193908No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG3402220.8040241904411155TruSeq Adapter, Index 6 (100% over 49bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA3199440.7561025318365429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT3011600.7117115447950055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2657110.6279372635111758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2176510.5143602385316036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2071420.48952501265748116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1982620.46853949493341546No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1843490.4356598205984011No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1830560.4326041590649307No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1597160.37744627801992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1574600.3721148221656979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1519710.35914303086081084No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1407690.3326700838399792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA1338230.31625506062924036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1335350.315574449243595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA1132890.26772841412631615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1102380.2605181872596355TruSeq Adapter, Index 6 (100% over 35bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1095130.25880484262563236No Hit
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA1050070.24815611032105575Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1043740.2466601832130227No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1041050.24602447327295804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1010380.23877643466263038No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT984600.23268401746751308No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT970140.2292667811354186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC946620.2237084548193147Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC937360.22152010015574658Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT922570.21802487710238022No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT919820.217374987758448No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA900340.21277140796942995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG896870.21195136577908638No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC875180.20682551128094465No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA831730.1965572596468156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA817100.19309984833709623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT802130.18956208707212702No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT791380.18702161054584657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC741920.1753330552909784Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA729120.17230811579922115No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA714920.16895232355055298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA712280.16832842978037804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC708500.1674351273367185No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC695290.16431329525186592No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACGTCT690010.16306550771151607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT672140.15884240859294563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC667120.1576560651360221No Hit
TTTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG658900.15571348680615923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT624110.1474917957969222No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT580530.13719282212108Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC572810.13536840549011395No Hit
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC572750.13535422608624634No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTC554260.1309846064610439No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG549340.1298218953438997No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC544220.1286119195471968No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA543420.12842286082896198Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTG541990.12808491837011723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA538780.12732632026319998Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA537590.1270450954198257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG519280.12271801400622609No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT498990.11792301226499528No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTAGATCGGAAGAGCACACGTCTG474620.11216381106076688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG466800.11031576209002145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA461040.10895453931873068Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTG460470.10881983498198838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT460140.1087418482607165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC460090.10873003209082684No Hit
ACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAA459170.1085126145648568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA455940.10774928998998368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG450430.1064471480681413Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT442940.10467708581866773Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATTAGTCAGCGGAGGAAAAGAAACTAGATCGGAAGAGCACACGTCTGA442870.10466054318082219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA441400.1043131477860657Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA437940.10349546882970007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTG430060.10163324045508702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)