FastQCFastQC Report
Wed 5 Jul 2023
SRR6880626.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880626.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45561755
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG28475966.2499699583565205No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC23701305.202016471929143No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC14054563.0847275307985833No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG7370331.6176571776043307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC6800191.4925215238087295No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG6046851.3271767077453447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT5031811.1043933667612233TruSeq Adapter, Index 3 (100% over 35bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3626540.7959614373941479No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2789570.6122613143413813No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2574400.5650353020861466No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2574200.5649914056207888No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2504940.5497900596673679Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2425600.5323763318599118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC2401970.5271899644778828No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA2325110.510320552840864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2180150.4785043947494999No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG2169790.4762305578439636No Hit
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT2037610.44721938388896565Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1966700.4316558920963426TruSeq Adapter, Index 3 (100% over 49bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1710650.375457442321965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1556930.3417186190479274Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1551640.3405575575392124Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1536210.33717094523685487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1532660.3363917829767532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC1453000.3189078208247246Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1448140.317841136716529Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1359390.29836208021398647No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1308880.2872760278878634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1303500.28609521296973744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1291500.2834614250482669Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1245360.2733345104902127No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1233650.270764372443511Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1201540.2637167949303094TruSeq Adapter, Index 3 (100% over 37bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC1105890.2427233103729213No Hit
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1088590.238926266119468Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC1083190.2377410615548062No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1072670.2354321074769837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT1018080.22345056725756066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC971520.21323147012225493Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC954220.2094344258688016No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA947660.20799462180506437No Hit
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA934500.20510623438451833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA932820.20473750407551247No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC881690.19351537270678007Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC877380.19256940387831856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC868480.19061601116989457No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT836830.18366939552701603Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG827240.1815645600131075Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT824800.1810290231357418TruSeq Adapter, Index 3 (100% over 35bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT781490.17152324356250107No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT774560.1700022310378518No Hit
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA772950.16964886449172117Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC764100.16770644589963665Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG763040.1674737946332401No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT753520.1653843228822068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT749050.16440323688145903No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA718530.15770463626785228No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC704460.1546165199299281Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG690850.1516293654623269Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA672510.14760405958901276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT667700.14654834959715665No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA663070.1455321464241226Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT654620.14367752076275378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA644750.14151123019734424No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT640980.14068378182534894Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA640650.1406113526575085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA640250.14052355972679278Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA630310.13834190539850802Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT616740.1353635302239784Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA604540.13268584583715004Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC593040.1301617990790741Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC591330.12978648430026454No Hit
TAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACTCC577080.12665886114351826Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA560490.12301764934208527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA551290.12099841193562452No Hit
AAGTACGCACGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT544470.1195015424669221Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAACTC538970.11829438966958143Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG537510.11797394547246919Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT533710.11713991263067017Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT533530.11710040581184812No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT533080.11700163876479297Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTT523390.1148748550182055TruSeq Adapter, Index 3 (100% over 35bp)
ATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA521840.11453465741168223Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG513020.11259882328940138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA512480.11248030283293521Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG511880.11234861343686169No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG505070.11085393879142715No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT504570.11074419762803255Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA498480.10940755025788626No Hit
CAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC466780.10244996049866824No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)