FastQCFastQC Report
Tue 4 Jul 2023
SRR6880627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44328446
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC12356072.7873907422786717No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA8706141.9640074908107537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT8293521.87092504889524Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG8062091.8187170378135973No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG4758661.0735002982058068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4665531.0524912152345698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA4426200.9985010528002719Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3949610.8909876967038276No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC3666010.8270107190312965No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT3605890.8134483216488122No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA3232910.7293082189256082Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA3001720.6771543491508816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC2422480.5464843049088615No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT2243460.5060994017250232No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2131930.48093948522355146TruSeq Adapter, Index 7 (100% over 49bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2054960.46357591691799893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1848140.4169196456830451Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG1507650.34010892238360896No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTAGATCGGAAGAGCACACGTCTG1489590.336034789038172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC1363900.30768053542865004No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1343400.30305596546289937No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC1339570.3021919604400298No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA1314040.2964326789168292Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1282600.2893401677108194Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC1280530.288873198938668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1267440.28592024182395204No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA1265360.28545101716401244Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1264740.2853111521211459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA1238070.27929469939009366Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACGTCT1181000.26642034778300144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1176850.2654841543509105No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT1130030.25492208772669356No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT1090000.24589176891064488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC1065060.24026558476694626No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1062850.23976703356576048TruSeq Adapter, Index 7 (100% over 35bp)
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG1016090.22921850226827264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC1013690.22867708919911156Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT1001470.2259203943219665No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA999130.2253925165795345No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA997280.22497517733872288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC976770.22034835148518403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA975200.21999417710244118Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA951430.21463193182995857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT938460.21170604536870072No Hit
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA912000.20573696628120014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATTAGTCAGCGGAGGAAAAGAAACTAGATCGGAAGAGCACACGTCTGA910530.20540535077633895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT871100.19651038522758052No Hit
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA867810.19576819814527222Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG772390.17424251687054404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG770260.1737620127716636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA733450.16545808982340596Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT733190.1653994367409135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC691100.15590440504050154Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC684760.15447417218280107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC681140.15365754080348318Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA681080.15364400547675414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT679500.1532875752062231Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA677260.15278225634167278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC674880.1522453550480881No Hit
AAAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGA647810.14613866680550905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACT647570.14608452549859294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG642650.14497462870681277No Hit
ACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAA637610.14383766126157455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC627530.14156372637109815No Hit
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA627110.14146897908399494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA618550.13953793913732054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACAGATCGGAAGAGCACACGTCTGA605310.13655114370578206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTG594480.1341080172311928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA583720.13168068197112076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA568170.12817277646051478Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA567590.12804193496880084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG556630.12556948195296536No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT552490.12463554440866255No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG550850.1242655788114025Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATAGCGTATATTAAAGTTGCTGCAGTTAGATCGGAAGAGCACACGTCT546440.12327073229681906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGAAGAGCACACGTCTG545270.12300679342560304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG542910.12247440390759469Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCT537280.12120433908285438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA529320.11940865240347022No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT516390.11649178949336506No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG510500.1151630715861323No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA501410.11311246958668482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT500380.11288011314450319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCACACGTCTGAA499810.11275152754057745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACACGTCT494320.11151304514487154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA494240.11149499804256617Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA476020.1073847704925185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT473200.10674861013625428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC467890.10555073372073542No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTC461890.1041972010478328No Hit
AAGAATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGAAC460780.10394679750334582Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC457110.10311888668508705No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTC455300.10271057099542809No Hit
ATGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT453630.1023338377348035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCATGTCTAAGTACGCACGGCCGAGATCGGAAGAGCACACGTCTGAAC449590.1014224590683824Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTAGAGGAAGTAAAAGTCGTAACAGATCGGAAGAGCACACGTCTGAAC446690.1007682516098128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACG443810.10011855592681955No Hit
TCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGAA443800.10011630003903137Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)