FastQCFastQC Report
Tue 4 Jul 2023
SRR6880628.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880628.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21966925
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC17053367.763198535980799No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT5375342.4470152285765985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT4268021.9429301096990135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA3689201.6794339672029652No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC3580051.6297456289398722No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3527861.6059871830035384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG3385801.541317230336062No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2908531.324049679233666No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2578891.1739877110701658No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC2456361.118208397397451No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT2228621.0145343510755376No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1998750.9098906651704779No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC1702840.7751835998893791No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC1509440.687142146659125No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC1470580.6694519146398505No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1328940.6049731585098962No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1322980.6022599885964922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1235130.5622680461648593Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1110240.5054143900432126No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1095270.498599599170116No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT987610.4495895533853737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG979930.4460933881278331No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC843470.3839727226273135No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA819040.372851457361465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA796510.3625951288129768No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC789900.3595860594962654Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT756480.3443722778677489No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC740310.33701121117316146No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA700460.3188703016011572Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC687110.312792983087073No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT658460.29975064784898203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG622050.28317572896525117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA613300.27919246776688134No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA561530.2556252183680693Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC548780.24982103776473039No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG531070.2417589170992299No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA524710.23886365524532904No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT505570.2301505558925521Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG505490.23011413750445273No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC504370.2296042800710614No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC495600.2256119142756667No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC492940.22440100287136228No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG466390.21231465032088012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA455100.20717510530035496No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG452430.20595964159753813No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG442700.2015302551449509TruSeq Adapter, Index 1 (100% over 49bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC435140.19808871746955936No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT433460.19732393131947235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT429170.195370995257643No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG421730.19198408516440058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC397930.18114961470483465No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACA391520.17823159135837174No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA383730.1746853508171945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA383560.1746079617424833Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG377500.17184926884395518No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT365100.1662044186885511No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT354410.16133801157877128No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC347410.1581514026200754No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA346890.15791468309742943Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG337100.15345798285376766No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACG335960.15293902082335148No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT329370.14993905610366495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC324300.14763104075786665No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA322240.1466932672643076No Hit
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT320750.14601497478595662No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA317680.14461741914264287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA315640.14368875024610864Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG305150.13891338910657727Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT304760.1387358494645928No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA302310.13762053632904925No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC302050.13750217656772626No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT300700.1368876162685492No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCAGATCGGAAGAGCACACGTCT300460.13677836110425104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT300070.13660082146226657No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC295750.13463422850489998No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA295490.134515868743577No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG295110.13434288140010492No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC293680.1336919027128285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC293110.1334324216976204No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA290030.13203031375579422No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA288480.1313247074863687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG281320.1280652617514741Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACAC274280.12486044359872855No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA273960.12471477004633102No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT273140.12434148156831237Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG270300.12304862879078432No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC269540.1227026541038402No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA263520.11996217039936176Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT253870.1155692023348739Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT253460.11538255809586458No Hit
TCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTC253290.1153051690211534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC252710.11504113570743286No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG249750.11369365534775577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC243480.11083936418046676No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA242430.1103613728366624No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA242220.11026577456790153No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG240990.10970584185087352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA238310.10848582584954426No Hit
CCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACTC237880.10829007701351008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGT235160.10705185181813111No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC233340.1062233334888702No Hit
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC230780.10505794506969No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT229450.10445248936753779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAG228520.10402912560588248No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC226480.10310045670934825No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCAGATCGGAAGAGCACACGTC225720.10275448202240413No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT225680.10273627282835446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)