FastQCFastQC Report
Tue 4 Jul 2023
SRR6880629.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880629.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9107030
Total Bases455.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5555566.10029834095199No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2635202.8935887989827638No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2034762.2342739619832153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1944502.1351637141856346No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1719131.8876955494821033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1252521.375333121775156No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1134431.2456640639154586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1026491.1271402422084917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG882130.9686253366904467No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT861700.9461921175180052No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG803370.8821426963565508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG781560.8581941642884672No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC733130.8050154660740109Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA709730.77932103001747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG635500.697812568971443Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA632850.6949027289906808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA577550.6341804078827017Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC461250.5064768645760472No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA446920.4907417676234733Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT443560.4870523101384315No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT403610.443185099862414No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT400910.44022035724050546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA400110.43934191498216213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT394760.4334673323794914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA368760.4049179589833349No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC340980.37441405156236446No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA316040.3470286141585127TruSeq Adapter, Index 6 (100% over 37bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC312980.34366857252034966No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC312200.34281209131846496Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC303760.3335445254929434No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC300920.3304260554758247No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG298680.32796641715246355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT284020.31186896276832293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC277040.3042045540642778No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT275700.3027331632815528Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT272960.29972449854672706No Hit
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG259690.28515333758645794Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC245390.2694511822185718TruSeq Adapter, Index 6 (100% over 35bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT240650.2642464118378879No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA233940.2568784773960336TruSeq Adapter, Index 6 (100% over 38bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT231870.2546055080525704No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA230050.2526070519148394No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC222790.24463518842037413No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG222130.24391047355724094No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT218090.23947434015260735No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG217220.23851903419665907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA205450.22559495247078354No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG203530.22348669105075966No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA202870.2227619761876265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT200500.22015959099728452No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC197990.2174034784117325Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG194610.21369205987023213No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC185640.2038425260485581Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC184060.20210760258833013No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC180950.19869265830902064No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG180540.1982424566516197No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT176170.19344396581541953No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA175090.1922580687666561Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC174040.19110511330258054Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC164140.18023439035558245No Hit
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT162340.17825789527431007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC158050.17354724866394422No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT156540.1718891889013213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG155290.17051662287265992No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA152820.16780443240002502Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG148610.16318163001549355Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG148130.16265456466048756No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA144580.15875647713908925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG142960.1569776315659441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG140740.1545399542990415No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC140330.15408975264164057No Hit
TTGGCGCACTGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA139060.15269522555652063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC136110.14945596972887978Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA135920.14924733969252324Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC131340.14421825776350797No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG129460.14215391845640127No Hit
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT129240.14191234683535686Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT129210.141879405250669Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC128600.14120959302868225Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC126440.1388377989311554No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG125450.13775072663645557No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT123660.1357852120834125No Hit
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT122320.1343138213006875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT120700.13253497572754236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA115910.12727530270571194No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT115870.1272313805927948No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG113260.12436546272494985No Hit
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC110700.12155444749825134No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAA109720.12047835573178083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT108930.11961089400166684Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC107400.11793087318258531Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG107260.11777714578737526No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA107210.11772224314622878Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGGAGATCGGAAGAGCACAC105820.11619594972235735No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA102540.1125943364631499Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT99670.10944292486134337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC99660.1094319443331141Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT99160.10888291792164953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG94790.10408442708544938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG94660.10394168021846859Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTC92770.10186636038313258No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC92720.10181145774198613No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC92450.10151498347979526Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)