FastQCFastQC Report
Tue 4 Jul 2023
SRR6880630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33919319
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC14015254.131937318670814Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG11900453.5084578201584766TruSeq Adapter, Index 2 (100% over 49bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC7404152.1828710653064705No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG7341142.164294631033129Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT4112491.2124329500836972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC3691771.088397440998152Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2920900.8611316754325168Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2887990.8514292400740711Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA2612070.7700832672967285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2404010.7087435924052602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2337450.6891205569309926Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG2150320.6339514068663937TruSeq Adapter, Index 2 (100% over 38bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA2057590.6066130042292417Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1977570.5830217287086454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1751430.5163517581234458No Hit
TCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACACGTCTGAAC1726720.5090668241305198Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1683740.49639557916832Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1621250.4779724498596213Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA1534070.45227028290279064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1495560.4409168710020387Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1415990.4174582632393062Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1372920.4047604847255335Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC1269350.3742262632100603Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC1215930.3584771262654182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1148650.3386418223785684No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1141330.33648376018398246No Hit
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1050510.3097084584746528Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAGCCACGATCACAAGAAAGACGAGATCGGAAGAGCACACGTCTGAACT1045950.308364091861632Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1043950.3077744573822369TruSeq Adapter, Index 2 (100% over 37bp)
TCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACACGTCTGAA1009370.2975796772334964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA1008340.29727601547660787Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC955390.2816654426346237Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG950820.28031812784920596No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC924650.27260276068632155No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG919540.2710962445914672Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA878020.25885543279922574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA862140.2541737350328289Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG846460.24955100071437164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT781940.230529392409087No Hit
ACCTGGTTTCAGCTCTCTCACTGAGATCGGAAGAGCACACGTCTGAACTC780990.23024931603137433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT773590.2280676684576126TruSeq Adapter, Index 2 (100% over 37bp)
TAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA773190.2279497415617336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTTAAATCCTTTAACGAGGATCCAGATCGGAAGAGCACACGTCTGAAC772060.22761659808087537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGCCACGATCACAAGAAAGACGTGAGATCGGAAGAGCACACGTCTGAA760500.22420851078997195Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA734930.2166700339709061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG696410.2053136738977572No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG670230.19759535856247587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATCTCAACCTATTCTCAGATCGGAAGAGCACACGTCTGAACTCC662200.1952279761277047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA657820.19393667661782948Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGA647110.19077918398066895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC632690.18652791938423055Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAACT621740.1832996706095426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC604780.17829957022427248Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC601170.1772352799889644Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGT598560.17646580699335385Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC592940.17480893410625373Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT574800.16946094937814052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC559970.16508880971342615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGAAC559480.16494434926597434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGGATATTCAAAAATATGCCAGAGATCGGAAGAGCACACGTCTGAACT544780.16061053584242066Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT534440.1575621255839482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT534430.15755917741155123TruSeq Adapter, Index 2 (100% over 37bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG524250.15455793791143035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT523290.15427491336132074No Hit
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC522250.1539683034320353Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAA515700.15203725051201647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA515410.1519517535125042Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC506240.14924827942447783Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT497170.14657428706042122Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC483410.14251760184218323Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA482440.1422316291196766Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC474620.1399261583052419Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTGAACT467150.13772387352470136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAA445530.13134992480244076Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA437830.12907983205676976Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT434990.12824255109602878Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA416110.12267640161053942Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC415210.12241106609481164No Hit
TCGACAGAGTGGTTCATTTCCACCTTTCGGAGATCGGAAGAGCACACGTC414830.12229903554372658No Hit
TTGGGGAAGGTGGCTCGGGGGGGAGATCGGAAGAGCACACGTCTGAACTC411540.1213290868251217Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG402030.11852537487559818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT401270.11830131377342805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC393210.11592508682146596Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG383520.11306830776879688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG382600.11279707590827516Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT377010.11114904753836596No Hit
AAACTTCTTAGAGGGACAAGTGGCGAGATCGGAAGAGCACACGTCTGAAC373240.11003758654470627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT373230.11003463837230931Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATATGCTTGTCTCAAAGATTAAGCCAGATCGGAAGAGCACACGTCTGAAC367990.10848979603629424Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTAAACACAGAGGTTTAAATCCTCTTAGATCGGAAGAGCACACGTCTG364310.10740486859420734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA361200.106487986978748TruSeq Adapter, Index 2 (100% over 36bp)
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC360920.1064054381516327Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTCGCTTCTGGCGCCAAGCGTCAGATCGGAAGAGCACACGTCTGAACT351080.103504436513009Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCA349250.10296492096436252Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)