FastQCFastQC Report
Tue 4 Jul 2023
SRR6880631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25646167
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA6914712.6961962775957904No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4259211.660758896251436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT3570891.3923679121328345No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC3522211.3733865181490863No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC3216541.2541991167724986No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3092761.2059345944366657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC3078281.2002885265466767No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC2606921.0164949795421672No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2273450.886467751691705No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2260960.8815976282147737No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC2232060.8703288877437318No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA2145410.8365421624213865No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1784840.695948053367975Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT1772350.6910779298910438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1754580.6841490192277075No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1714790.6686340301847056No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC1558000.6074981887156861Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1537400.5994657993141821No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1500630.5851283741543132Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1273440.4965420368665618Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA1098590.4283642074076801Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1062430.4142646345553314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1019540.39754088788394776No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT1015530.3959773014033637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA980690.38239242534761625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA970620.3784659126644539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC946100.36890502974577055Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC883930.3446635904694842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC854430.3331608969090781No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG840670.3277955727263259No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT832550.3246294075835972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT780430.304306682554161No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC774970.30217770944094685No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT768420.29962372154872113No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG751370.292975554592622No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC743650.28996535817613606No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC740450.28871760836619365No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT733160.28587507833041875No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT712140.27767892176635983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGCACGGAAATGTCCCGAGAGCTACATGAGATCGGAAGAGCACACGTC665480.2594851698501378No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT642320.25045458060068004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA632690.24669963351638471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG598900.23352417536702466No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT598380.233321416022909Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT593110.2312665280546602No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC580010.22615855227020865No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA574570.22403737759330664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG573210.22350708392408114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG556860.21713186223890688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG513880.20037302260411857No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG505160.19697290437202564No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC499950.19494141171271323Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC490980.1914438130267186No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC460710.17964087966829506No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC453260.17673596214202303No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT440710.17184244335615534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT439680.17144082388608012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA434150.1692845562457735No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT416690.1624765213452755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT410920.16022667246922317No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT410790.16017598263319427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC405830.15824197042778362No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG387240.15099332387564973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG379270.14788564700526202No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG378450.1475659111164643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACGTCT374140.14588534809119819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGT367650.14335475550790885No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA356330.13894084055523775No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG355560.13864060075722037No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG350920.13683136353280395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC341880.13330647031971676No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA341460.13314270315716184No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACG331710.1293409654549937No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA329430.1284519437154098No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG318700.12426808263394681No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT309410.1206457089669579No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTCTGAAC308160.12015830669744917Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACG294170.11470330049710743No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC289860.11302273747184131Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG284100.11077678781394507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG282390.11011002150925711TruSeq Adapter, Index 1 (100% over 49bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA282040.10997354887379467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC279640.1090377365163379No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG270400.10543485894012933No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA270170.10534517692253972No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCAGATCGGAAGAGCA263780.10285357652081108No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC261370.10191386494519823Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC259370.10113402131398427Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCC258840.10092736275171257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)