FastQCFastQC Report
Tue 4 Jul 2023
SRR6880632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17302132
Total Bases865.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5058262.9234894289328044No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG4657082.691622049814439No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2961721.7117659257252227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2782901.6084145005944932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2268811.3112892677041188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC2157271.2468232238662842Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA2112561.2209824777663238Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1893801.094547192218855No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1851861.0703074048909116No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1788281.0335604883837437Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1724330.996599725398003No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1588910.9183319142403954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1584160.9155865878262864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1508210.8716902633733229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1507810.8714590779910822No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1398290.8081605203335636Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1285930.7432205464621354Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1204650.696243676790814TruSeq Adapter, Index 2 (100% over 37bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1183420.6839735126283859Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1127280.6515266442308959No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1101490.6366209667109233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC1088170.6289224934823061No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1081260.6249287660040971No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT1067810.6171551575262516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1064950.6155021820432303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG1004400.580506494806536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC914650.5286342746662666No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT848730.4905349236729901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG823580.4759991427646026No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG820140.47401094847733216Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT787890.4553716270341713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG754250.4359289363877238No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA734320.42441012471757816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC692770.40039574313731974No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA676530.3910096166183451Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA663260.383340041562508No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG655100.3786238597647966No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG642270.3712085886294244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT642180.3711565719184202Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT620090.3583893591841745Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG611180.35323970479476174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC583900.3374728617259422No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC582380.33659435727342735No Hit
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG572750.33102856919598117Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT570110.32950274567319215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT557280.32208747453781994Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCAGATCGGAAGAGCACACGTCT519790.3004196245873052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA488570.2823756055034143No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC480930.27795996470261586No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC464790.26863163452920136No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA449260.25965586206370406Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT432360.24988827966403215No Hit
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC414860.23977391919099914Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT414250.23942136148308196No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG411490.23782618234562078Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT401610.2321159034042741Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG395060.22833024277008176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA362670.2096100064431366No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCAGATCGGAAGAGCACACGTCTGAA362490.20950597302112828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC347560.2008769786289921No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT343370.19845531175002018No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC334260.193190064669487Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT334220.1931669461312629No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA312870.1808274263541626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA309650.1789663840271245Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC297950.17220421159658245No Hit
TTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCC296710.1714875369116361Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT286630.16566166527916906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG284390.16436702713862084No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC281700.16281230544305175No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA271570.15695753563780462Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC271370.15684194294668427Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT266600.15408505726346325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC261750.1512819345037941Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC259580.150027753805138Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG257060.14857128589702123TruSeq Adapter, Index 2 (100% over 49bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG250450.14475094745549277TruSeq Adapter, Index 2 (100% over 38bp)
TTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACT247790.14321356466359175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG244500.14131206489466153No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA240490.13899443143769796Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT235050.1358503102392237TruSeq Adapter, Index 2 (100% over 37bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC234430.13549197289675052Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTC231320.13369450654982867No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA221280.1278917534555857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGTCGGGGAGCTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT220090.12720397694341945TruSeq Adapter, Index 2 (97% over 37bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT219670.12696123229206666No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTGA217250.12556256072951008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT211620.12230862647447147No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA207220.11976558726982316Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC203730.11774849480977256No Hit
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC201070.11621111201787154Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT196090.11333285400897414No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA192840.11145447277826802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC191960.11094586493733836No Hit
TTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAAC191070.11043147746185268Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG190960.11036790148173647No Hit
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT188860.1091541782249725Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTAGATCGGAAGAGCACACGTCT186570.10783064191164418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGGCGCCGCCGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA186230.10763413433673955Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG186210.10762257506762751No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT182310.10536851759078016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT177010.10230531127609015No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA174650.10094131752086967Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT174360.10077370811874514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)