FastQCFastQC Report
Tue 4 Jul 2023
SRR6880633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39379875
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG14480613.6771599706702984Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC8026792.0382974806293825No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG7553541.9181218833223823No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA7507811.9065093528102868Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT6773691.7200892587901817No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6211891.577427556588232No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC5639651.4321147540463244Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5400401.3713603712556224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT5294201.3443922815905331Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA5110171.2976602896784208Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG5106751.2967918257739517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC4838261.2286123305368541No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA4450581.1301661064185704No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG4395791.116252908370075No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3916470.9945359146010494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC3829780.9725221321804601Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT3564250.9050942899133123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA3387840.860297296525192Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC3247650.8246978945463895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2796120.7100378048432099Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT2710930.688404927643879No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG2642950.671142303016452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2564030.6511016096419808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC2485830.6312437507737138Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT2435730.6185215163836858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2432710.6177546272048857TruSeq Adapter, Index 2 (100% over 49bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA2286830.5807103247534432Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2218690.5634070702357487No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2056710.5222743850761334Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1912480.485649078368075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1784860.45324166214341716No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG1734060.4403416719834687No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG1630850.4141328533927545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1579760.40115922155669614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1570500.3988077666574614Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA1511790.3838991362974108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC1474460.3744196750243621No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1465400.37211900748796184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1407570.3574338415243827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG1381770.3508822717187396No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1319200.3349934452559842No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG1261060.32022955888001164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC1149880.29199686388034496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA1108140.2813975412567968No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG1104920.2805798647151623TruSeq Adapter, Index 2 (100% over 38bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1101850.27980027869565355TruSeq Adapter, Index 2 (100% over 37bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA1098040.2788327794336574Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1076820.27344424023692304Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG1041430.26445741638336845Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1028040.26105720244160247No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG1025990.26053663197254945No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT973800.2472836696409016No Hit
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC953890.24222778767073283Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC933960.23716682696428062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT929460.23602411130050566Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG909600.2309809261710455No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT895880.2274969130806027Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC890650.22616882354248202No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC874240.22200172042191602Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG868670.2205872923669768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA847140.21512003275784902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT843170.21411190360558535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG841920.21379448258787007No Hit
TTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAAC832140.21131098054526584Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA830810.21097324458241679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA830610.21092245721958236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC825010.20950041106021794Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC784740.1992743755535029No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT756120.19200670393189415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA729390.18521897288907088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACACGTCTGAACTCC717120.1821031681791778Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT691840.17568364551690424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAA687950.17469583130977434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC667180.16942156367941746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC620730.15762619866111816Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC619730.15737226184694592No Hit
CCCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA617120.15670948676195645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG611770.15535092480613513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA571650.1451629798215459Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC566670.1438983744869683Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG565750.14366475261792983Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT565480.14359618967810334No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA555360.14102634911868053Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA549080.13943162592567904Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC546990.13890089798405914No Hit
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC531940.1350791489307673Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG531490.1349648773643898No Hit
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT516000.13103139611286221Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC507070.12876374036230434Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCAGATCGGAAGAGCACACGTCTGAA487660.12383482679922167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG484460.12302222899387061No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG482750.12258799704163613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA481920.12237722948587318Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG480240.12195061563806386Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG477220.12118372645926379Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC474040.12037620739019612Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT465140.11811616974406343Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT454720.11547014814038896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC447610.11366465739162453No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC444840.11296125241636751Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGTCTCTCGGGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCC432510.10983021149762409Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC422740.10734924882316159Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA416130.10567072648148325Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC412470.10474131774161294No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG406020.10310342529020217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT401210.10188198921403382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACA397560.10095511984230524No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)