FastQCFastQC Report
Tue 4 Jul 2023
SRR6880634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26906469
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC13892485.163249031301729Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG6428772.389302736081795Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC5591852.0782548613123484No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT4015021.4922136382889928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG3645421.354848902693252No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA3584931.3323673202901503No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT3563481.3243952597421833No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC3098271.15149631859907No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3081901.1454122798498756Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2821641.0486846118678748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2821251.048539665312457TruSeq Adapter, Index 6 (100% over 49bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2792151.037724422331299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA2225350.8270687617910771Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2061760.7662692566609167Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA1867270.6939855244476709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1864450.6929374493546515No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1828080.6794202539173758No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1662920.6180372459872011Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1544730.5741110065389852No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1541580.5729402843606123Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT1526250.5672427697591981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1474200.5478979794784667Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1410590.5242568246320243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAAC1375330.5111521693909372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1333220.49550165798418216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC1331820.4949813370160165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1325400.4925952937191424No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1276450.4744026427250636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1271610.472603818806548No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG1146000.4259198782270539No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGA1099900.4087864520610267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1082950.40248685176787785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1063530.395269256623751Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA1058630.393448133235171TruSeq Adapter, Index 6 (100% over 37bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1013880.3768164451455894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC976970.3630985544777354No Hit
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA972550.3614558268496695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG949590.35292256297175223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT941450.34989726819970324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA894290.3323698847292077Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT863660.3209860052614113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA842910.3132741051975271Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC780030.2899042605701997No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA771820.28685294974974235Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC743560.2763498993494836No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT735110.27320939064876926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT726220.26990535250091713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC717130.26652698278618425No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC712580.26483593963964575No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCAGATCGGAAGAGCACACGTCTGAA697770.2593316871121216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA690940.25679326410314185Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG680660.2529726215654682No Hit
TCGGGGTCGGGGTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCT663270.2465094918251815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG662280.2461415505691215Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA650350.24170767260468107No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG641150.23828842052816368No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACG635220.2360844895701476No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG634670.2358800777612254No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGT605020.22486042297114495No Hit
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA584730.21731948551108657Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT552190.20522573957957843Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG551830.20509194275919299Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540680.20094795790558767No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA526390.19563696745195364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC515810.19170482756395868No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG500490.18601102954088847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG494260.1836956012325512Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA492600.18307864922744044TruSeq Adapter, Index 6 (100% over 38bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA487780.18128725846561286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG482450.17930632220823922Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG448240.1665919076932763Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA446330.16588204122956454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT443430.16480423350979276No Hit
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGT442360.1644065596269804No Hit
GTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC431030.1601956763631824Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA430470.1599875479759161Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTC426680.15857896478352473No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA421980.15683217296182567Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA401590.14925406971832686Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC398170.14798299992466496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG397520.1477414223323023No Hit
TTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAAC396570.14738834738961845Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT384600.14293960311180184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT383500.14253077949395737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC383000.14234495057675536No Hit
TCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACAC382700.14223345322643413No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA377220.14019676829389988No Hit
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG372290.13836449517028787Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAA349480.12988697996753124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA343470.1276533163827628Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG334390.12427866324637395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT333920.12410398406420403No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTC333200.12383639042343313No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA332950.1237434759648321Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG332630.12362454545782281Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC331490.12320085552660216Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT314440.11686408945001292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC301650.11211058574798499No Hit
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT299350.11125577272885566Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT298890.11108481012502978No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC296570.11022256394921236Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC294860.10958702905238141Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTG288000.10703745630836956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG285350.1060525630471988Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC280550.10426860544205929Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG279590.1039118139210314Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC275560.10241403284838305Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT275500.1023917333783188Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA275180.10227280287130949TruSeq Adapter, Index 6 (100% over 37bp)
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTC274450.10200149265219453No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC273250.10155550325090966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTGAACTC272950.10144400590058845Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT271600.10094226782414296Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)