FastQCFastQC Report
Wed 5 Jul 2023
SRR6880635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63462770
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG25413594.004487985633152No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC24689943.8904605014877225No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG16974142.6746610650622404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC11615811.8303345410230283No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC8533511.344648208705671No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG8088191.2744779340706371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA7305471.151142630553315Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG6428081.0128899195544097No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT5674710.8941793747735877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA4574250.7207769216502841Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG4128410.6505247092114006No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG3857700.607868203672799No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC3482800.5487941985513711Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT2868490.4519957133922771No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT2841900.4478058553069776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG2571660.4052234089372399Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC2413480.38029855929704925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2338940.36855309026063626No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2312750.364426261255221Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT2122230.3344055105063961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA2120780.33417703009181604Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT2052340.32339275452363647No Hit
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC2047720.3226647686509744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA1840410.289998372274012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA1823040.2872613344800424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1812900.28566354730497895No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1806560.28466453638881506No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1747640.27538035292187846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1485940.23414357740766753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1466070.23101260786442193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1465990.2310000020484451Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1412340.2225462267089823No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1396010.21997306452271154No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1271230.20031114305284814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG1244470.1960944976085979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC1190060.1875209670173552No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1172510.18475556613743774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA1157850.18244555035968332Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1136650.1791050091258229Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1090680.17186139212013596Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1087980.17143594583091787No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC1077270.1697483422170195No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1077030.16971052476908904Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC1072290.16896363017246174Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA1063020.16750293124614635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1037820.16353209921344436Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1031510.16253781547827176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA1015440.16000562219392567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAACTC974680.1535829589537299Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC973720.1534316891620079Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC959490.15118942964512894Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA957360.1508537997947458No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC917510.14457452771128648No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT905190.14263323205085437Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC898420.141566464873815No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA879480.13858203794130006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT859290.13540064513414715No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA848060.13363110371639939No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC846160.13333171558694965No Hit
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT838050.13205380099229833Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA835420.13163938479205997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT831510.1310232755361923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT823260.12972330076358154No Hit
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT814380.12832405519015322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA810310.1276827343023319Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCT796460.1255003524113429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG789330.12437685906240778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC782520.12330378897737998No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT781170.12309106583277094No Hit
GGGGGGGGGATGCGTGCATTTATCAGAGATCGGAAGAGCACACGTCTGAA766610.12079680732498756Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT742370.11697724508400753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT737540.11621616894440631No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTC731760.1153053987400802No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA730570.11511788722742483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC720430.1135201000523614Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC712170.11221854955275351No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA703420.11083978843028756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT690070.10873619288915375Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACTCC686040.10810117490932085Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA672280.10593297456130578No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC657690.10363398887253109No Hit
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA651970.10273267303018761Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC650800.10254831297152646No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCT649810.10239231599881315Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA648580.10219850157816937No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG644450.10154772632836542No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA642350.1012168236589736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC639860.10082446763669473Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA635530.10014217784694869Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)