FastQCFastQC Report
Tue 4 Jul 2023
SRR6880636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44552428
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC20825884.674465777712497No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG15714373.5271635476297725No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC8552511.9196507090477761No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG6382651.4326155243435892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG5391921.2102415607966417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC4596571.0317215483744229No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG3552590.7973953742767959No Hit
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA3052990.6852578270257235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT2840780.6376263040030051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2764160.6204285880895201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2573230.5775734601939091Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA2333490.5237627004301539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG2080930.4670744319479064No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1998520.44857712356327695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA1794270.4027322596200593Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1785490.4007615477208111No Hit
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC1735220.38947821205165295Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1642640.36869819979283736Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1432360.3214998742605005No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1332530.2990925657295266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1315050.29516909830368837No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC1314930.295142163744701Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1274670.2861056192044124No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC1257560.28226520000211885No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1168030.2621697744509009No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1157580.25982422327241067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1139580.25578403942429356No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1100230.24695174862299313Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1078420.24205639252702454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA1022170.22943081800165863Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1019850.228910083194568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1017850.22846117387811052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1011740.22708975591633299No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT1002460.2250068166879704No Hit
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC979430.2198376259089628No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT968330.21734617920262392No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA946990.21255631679602285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT940770.2111602088218402No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA911820.2046622464661185No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA887740.19925737829597076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT847140.19014451917188444No Hit
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA847010.19011534006631467Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT814910.18291034553717253Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC808150.18139303204754634No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC799730.17950312382526043No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC784690.17612732576550036No Hit
AAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAACTCCA781830.1754853854429662Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA759180.1704014874340855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG754410.1693308387143345No Hit
TTCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACAC753490.16912434042876406No Hit
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA741220.16637028177229757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG731210.16412349064342802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC701590.1574751436666931Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG700260.15717661897124888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG696670.15637082674820776No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGATCGGAAGAGCACACGTCTG695010.15599823201554805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA687760.15437093574338978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCACACGTCTGAA686220.15402527556971754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA677650.15210169914869734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA662950.14880221567273505Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT662440.14868774379703842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTG660420.14823434538741637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCACACGTCTGA639170.1434646839000559Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT636680.14290579180106636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC633250.14213591232334183No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC620970.13937960912029307No Hit
AAATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTC612950.13757948276129867No Hit
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT609230.1367445114326878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC608640.13661208318433285No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC603980.13556612447698696No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT597250.13405554462710764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA595300.1336178580435616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA592720.13303876502533152No Hit
AATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTCT580710.1303430645800045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCT564000.12659242724100245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC555770.12474516540378001Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCTG549830.12341190473390137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC548730.12316500460984978No Hit
AGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACT547980.12299666361617823Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACACG542530.12177338572883166No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT539240.12103492990325915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC537750.12070049246249834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAACTCC530670.11911135348223895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA523690.11754465996780245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA523090.1174099871728652Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC518930.11647625579463369No Hit
TCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACAC513700.11530235793209745No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG507710.11395787452930736No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC498720.11194002715183109No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG497390.11164150245638689No Hit
TAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACTCC496870.11152478603410797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT494370.11096364938853612No Hit
AAAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGA492560.11055738645714212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAGATCGGAAGAGCACACGTCT485390.10894804655764215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC482390.10827468258295597No Hit
TTCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTG480510.10785270782548595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATACCGTCGTAGTTCCGACCATAAACAGATCGGAAGAGCACACGTCTG478730.10745317853383884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG477900.10726688116750899Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG461450.10357460203964641Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC458020.10280472256192189No Hit
TAGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAA457580.10270596251230124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC456260.10240968236343932No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA453570.10180589933280404No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC452050.10146472825229638Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA451970.10144677187963808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT448930.10076442971862275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCTGA447010.10033347677482359Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA445750.10005066390545538Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)