FastQCFastQC Report
Wed 5 Jul 2023
SRR6880637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44063786
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG17512083.974256774032082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC6485031.471736904314123No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG6092131.3825707123759179Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4751371.0782936355037673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC4035680.9158722766128176Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3774050.8564969882524393No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA3673970.8337844596467494Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAG3192300.724472472701279Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA2957550.6711974318321172No Hit
TTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACACGTCTGAACTCC2483500.5636147561174157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2333660.529609507453581Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2307060.5235728042070648No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT2303580.5227830400229341Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2256560.5121121457879266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG2192690.49761724968435533Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC1910330.4335374177788536No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1776370.40313603556444294Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC1717280.38972593049539594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1687990.38307874861229585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG1686550.38275194963955206No Hit
ATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1609400.36524324078734405TruSeq Adapter, Index 4 (100% over 35bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1508780.34240816256687523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1344820.30519846842030324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1322450.3001217371562217Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1268750.287934858797653TruSeq Adapter, Index 4 (100% over 37bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC1184130.26873088027433684No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA1182840.2684381228612539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1118500.2538365632040787Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG1095990.24872805981764706No Hit
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1049520.23818198463472928Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1033530.23455315437488738Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1022580.2320681205196485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA1010280.22927671262746238No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT1009990.2292108989454515Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1008970.22897941633975802TruSeq Adapter, Index 4 (100% over 35bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG1000050.2269550782585954Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC992690.22528477239790518No Hit
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC961780.21826994167046834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGCGGGGGGCCCGGACAAGATCGGAAGAGCACACGTCTGAACTCCA949650.2155171142125645Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC938590.2130071165469077No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG917690.20826399256750205No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG886680.20122646746695805TruSeq Adapter, Index 4 (100% over 49bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT853640.19372824659233776No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG843490.19142476772195655Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA841830.19104804112837695No Hit
TGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAG835540.1896205650599338Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA749690.17013744574739903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCGTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACG700790.15903989729797616No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAG688190.15618040628646843No Hit
ACAGAGTGGTTCATTTCCACCTTTCGGGCGAGATCGGAAGAGCACACGTC655180.14868899372378033No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT639140.1450488162773848No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG634050.1438936726862281No Hit
ATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT627730.1424593883058528Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGT612580.13902119078011135No Hit
AAAAATCCGGGGGAGAGGGTGAGATCGGAAGAGCACACGTCTGAACTCCA600890.13636821856387918Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC595540.13515406960264376No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT591620.13426445017684135No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGA574750.13043590943365602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA572660.12996159703571544Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG569310.1292013355366241Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA566990.12867482608053699Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT562160.12757868785945903No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA560750.12725869719864744No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC559610.1269999813452253No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC539080.12234082654631628No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC523470.11879823490428172No Hit
TTATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAAC519930.11799485409628668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA517550.11745472801633523Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA502240.11398021949362226Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA496640.11270933459961884TruSeq Adapter, Index 4 (100% over 36bp)
TGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT495940.1125504739878684Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA492760.11182879292305932Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTG487960.11073946301391352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAA458160.10397653982796666Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA450290.1021904926644297TruSeq Adapter, Index 4 (100% over 36bp)
TCGGCGGCGCGGGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC441380.10016842402057781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)