FastQCFastQC Report
Tue 4 Jul 2023
SRR6880639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16935565
Total Bases846.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC7107794.196960656464665No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG3185951.8812186071146726No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT3183841.879972708321216Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2707091.5984645330699037No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2603421.5372501596492352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG2584471.526060689442602Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT2237591.3212372896918407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1603110.9465937510794591No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1585240.9360419921035997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1474940.8709127802940144No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1436150.8480083185887215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1403890.8289596479361628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1307430.7720025874542715No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1229260.7258452847602073Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT1091820.6446906259106207TruSeq Adapter, Index 7 (100% over 38bp)
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1085710.641082833669854Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT915920.5408263615651441Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA906000.5349688658158143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG903330.5333923019397345No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA876880.5177742815193943TruSeq Adapter, Index 7 (100% over 37bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA834300.49263192577277465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT834120.49252564056764564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC833550.49218907075140395Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC696620.41133555331634936No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA678230.40047674819233964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC603550.3563801975310537No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA563440.33269631098814834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT553770.3269864335792753No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG543130.3207037970094295No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT518730.3062962469808359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC493570.29143993719725325No Hit
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG488370.28836947571574967Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC468500.27663677001623505No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA453440.26774424118711126No Hit
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA449650.26550634714578464TruSeq Adapter, Index 7 (100% over 35bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA449250.26527015780105356No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC448510.26483320751330114No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT445660.26315035843209245No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA443170.2616800797611417Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC435560.25718657747763357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC434410.25650753311153185No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC414050.24448549546472173Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT409060.24153903338920193No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT407610.2406828470145519No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG400340.2363901056740652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG397230.23455373351878134TruSeq Adapter, Index 7 (100% over 49bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT395490.2335263098692013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC391620.23124117795892846No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC376980.22259664794177222No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT371400.21930180658277418No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG360260.21272393333201461Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG341510.2016525577977469No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC341320.2015403678589997No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA340370.20097941816526343Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA323900.19125432189596273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT323410.1909649899486672No Hit
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA319050.18839052609109883Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA305950.18065532505115714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT296720.1752052559214883TruSeq Adapter, Index 7 (100% over 38bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT295810.17466792516222518No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC282470.16679101051544487No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG280790.16579901526757448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT275090.1624333171051571No Hit
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC273510.1615003691934695Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA262800.15517639948829579Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG261510.15441468885153817No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA259230.1530684095865712No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC258990.15292669597973257No Hit
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT255350.1507773729426801Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA250990.14820290908511172Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC246100.1453154943457747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC242920.1434377890551629No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC242580.14323702811214153Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG234030.13818848086851546No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC232930.1375389601705051Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAG230070.13585020635567813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT228840.13512392412063015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG227090.13409059573743187No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC225070.13289783954654008No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG221320.13068356443968654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGC221250.13064223130435862No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC220930.13045327982857377No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA219800.12978604492970858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA216910.1280795769140268No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT211730.12502092489975977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG210650.12438321366898596No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC210340.1242001669268194No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG209400.12364512196670144Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGCGCACTGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA207820.12271217405501382Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT206150.1217260835407617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA202510.11957676050370919TruSeq Adapter, Index 7 (100% over 37bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG200170.1181950528370326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG196050.11576230258630285Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGAGGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT195770.11559697004499112TruSeq Adapter, Index 7 (100% over 38bp)
TGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAG192130.11344764700793862Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG191300.11295755411762169Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC190990.11277450737545514Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC190390.11242022335835858Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT187020.11043032812899953Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG184600.10900138259337673No Hit
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC184570.10898366839252188No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA183750.10849948023582326No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC183480.1083400524281298No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG180550.1066099654779749No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC179350.10590139744378177No Hit
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC177630.10488578326143828Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAAAGATCGGAAGAGCACAC177170.10461416551499757No Hit
AAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA176200.10404140635402481Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT175500.10362807500074547Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACTC172840.10205741585828403Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC172370.10177989337822506Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT172220.1016913223739509No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA171230.10110675374574159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA170980.10095913540528467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)