FastQCFastQC Report
Wed 5 Jul 2023
SRR6880640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36569991
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG17719014.845232256141381No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC13291293.634479975671856No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC10663692.9159673569512226No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG10024632.741217519030836Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG8754522.3939081636634802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA8155542.230118131557648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT6835761.8692266016691117No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA6783011.8548022065414234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT4281581.1707905533802292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT4029631.1018952670784086Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA3428650.9375583384748439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC3285780.8984907871593405No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2754260.7531475739220171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2601690.7114275745925122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT2557950.6994669481871078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2439760.6671480996536204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT2228700.6094341122479358No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2217290.6063140677283733No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2096220.5732076882381514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1839500.5030080537892394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1817750.4970605543764012No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1759890.48123883869700707No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT1696580.46392683006129265No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1655000.452556851873439Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1632180.4463167628343141No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1598290.4370496016802411No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1572060.42987705411248256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT1567720.4286902887124035No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1488150.4069320115501259No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1466020.4008806018027185Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1395440.3815806243977473Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC1297790.35487840289597006No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1296020.354394399495477Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT1216450.3326361223331994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACTC1205160.3295488916035008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT1164070.31831290305759163No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1080290.2954034087675876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA1079550.2952010570634267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT1052550.2878179543440413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1020520.27905940693285924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1011850.27668861061518996Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTG1011740.27665853130781465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1003920.27452016600168155No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC997730.2728275213412002No Hit
CCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAAC995310.2721657765789442Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG970120.2652776151900065No Hit
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG953580.2607547811537608Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG948270.2593027709522816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC880590.2407957934690222No Hit
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC872610.2386136764430705Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC867390.2371862765839893No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG864630.2364315594171188No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC845800.23128252889096967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA842390.23035007036233615Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT832030.22751714650408308No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC829540.22683626036440643No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC812420.22215482634381833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC784510.21452288571796474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA711020.19442717390879316Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT707870.19356581192486486Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC694180.18982230539788758No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG686610.18775230215397098No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC676580.18500961621784373No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC665530.18198801306787304No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA664960.18183214756601937Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA663690.18148486828995938Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC659870.1804402959792908Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG656750.17958713744282848Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACT653070.17858084788700113Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA651230.17807770310908747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT633890.1733361104737488Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC624560.17078483831182786No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG618200.1690457074490393No Hit
TATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT614890.16814059374529242Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG612950.16761010414249214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG611100.16710422488208979Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC610060.16681983870326902Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGA606150.16575065605020248Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT601680.1645283423777709Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCC591890.1618512840213715Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC589570.16121688408400212No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG584300.1597758118124776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG571550.1562893466394345No Hit
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA569960.15585456392373737Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT560100.15315836418991735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC559100.15288491594105122No Hit
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT555510.15190323672762185Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA536460.1466940475867221Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC516670.14128250674166148Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTC506950.13862458976268274No Hit
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG505740.13829371738155474No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC502040.13728195886075006No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC498790.13639325205193514No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA491900.13450919361724753No Hit
TGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAG481860.13176377319863164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGT479290.13106101119904567Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC476460.13028715265475455No Hit
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC462440.12645340820565146Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT454580.1243041049695637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA453030.12388026018382121Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA450370.12315288784183731Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC443350.1212332811347971Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA436500.11936016063006415Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC431540.11800385731568816Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA427390.11686904708289372Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC425570.11637137126995739No Hit
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA425400.11632488506765014No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT424060.11595846441416953No Hit
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTC414540.11335523708496399No Hit
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC409920.11209190617520251Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC407040.11130437521846806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGA406170.11106647524195452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT403450.11032269600503868No Hit
TTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACT402690.1101148753359004Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC401950.10991252363173948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACGTCT397170.10860544100215938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCAC384480.10513538272404827No Hit
TCTCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGT384410.10511624134662761Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380330.10400057249125383Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA377110.1031200691299049Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT375510.10268255193171909No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG372250.10179111064041553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA367150.10039652457119827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)