FastQCFastQC Report
Tue 4 Jul 2023
SRR6880641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22666588
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC7354203.244511260362609No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA6642312.930441052707183No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC6188922.730415358500362No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA4336441.913141933845535No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT3342481.4746286472405992No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2905141.2816838599616316Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2768351.2213351211042438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC2665101.1757834924250619No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2440701.076783148835634No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC2305391.017087353420815No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2154080.9503327099782287No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC2093200.9234737932325765No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1935600.8539441401590747No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC1656030.730604006213904No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT1416290.6248359920778548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT1264370.5578122300542102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGATCGGAAGAGCACACGTCTGAAC1230140.5427107070548068Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1224310.5401386393046894No Hit
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1205720.5319371402524279Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC1184200.5224429896550817No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1110220.4898046410866956No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1043350.46030306811064814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1021750.45077362327316306Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC885650.3907292972369728Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA864130.38123514663962654Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC860570.3796645529534485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC787180.3472864994060862Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT767040.3384011744511349No Hit
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC739470.3262378969432894No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA734800.32417759567518495Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA719230.31730845418816456Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG708140.31241579014891874No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC690240.30451870391785474No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA657650.2901407128412975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA651710.2875201155109891No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG638170.28154656536749156No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT635680.2804480321431704Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT633700.27957449969973425No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT617610.2724759456518114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACG614870.2712671179270564No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC610570.26937005251959406No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA589670.2601494322833238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA572740.25268028871394316Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT561280.24762438881405527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT557480.2459479124074607Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG543980.23999200938403256No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC535780.23637434976980215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG524030.231190508249411No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA516500.22786843789634326Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT503530.2221463592138349No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT499720.22046547102722297No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC487140.2149154517653914No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG480800.21211838323438886No Hit
ATAGCACGGAAATGTCCCGAGAGCTACATGAGATCGGAAGAGCACACGTC479520.21155367539216755No Hit
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGT478890.21127573325107424No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA474610.20938749140364663No Hit
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT465270.20526688886743785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC450910.19893157276251724No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG450290.1986580424014413TruSeq Adapter, Index 2 (100% over 49bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT441140.1946212636855622Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC421260.18585064501106208No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA420120.18534770208908374Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT419200.18494181832748716Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACACG409560.1806888623907577No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG402330.17749914543821063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA401420.1770976734566314No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTAGATCGGAAGAGCACACGTCT386440.17048882699063483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACACG377310.1664608718347905No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT371110.1637255682240309Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT366770.16181085569649917Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACAC364960.1610123235133581No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG359080.1584181968631538Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC351830.15521965635057206No Hit
ACGGCTGTCTCTCCAGCCACCTTTTAGATCGGAAGAGCACACGTCTGAAC350380.15457994824805568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT344800.15211817499837205Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG342460.15108581847431118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG328820.14506815053064007Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC323870.14288431942204974Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG320450.14137549065611463No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT311560.13745341822068677No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC310930.13717547607959346No Hit
TCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACG310670.13706076979914225No Hit
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA303130.1337342876660572Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC301710.1331078149035929No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCAGATCGGAAGAGCACACGTCTGAAC298250.13158133901758834Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG296630.130866630654777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTG287340.12676808701865494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC282270.12453131454985637No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG281960.1243945493693184No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT281640.12425337240876307No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG277600.12247101328175197Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG274540.1211210085964416No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA262860.11596804953617193No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT261320.11528863541349937No Hit
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA260080.11474157469134746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA250030.11030773577390653No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC249710.11016655881335116No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA246640.10881214234802344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG246120.10858272978712102Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCC243440.10740037274247011Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA241260.10643860469868689No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC240170.10595772067679529Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC239560.10568860209573669No Hit
GTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC238090.10504007043318563No Hit
ACGGCTGTCTCTCCAGCCACCTTTTTAGATCGGAAGAGCACACGTCTGAA237930.10496948195290795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC235950.10409594950947182No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG234500.10345624140695546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG232910.10275476838419616No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGT226740.10003270011348864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)