FastQCFastQC Report
Tue 4 Jul 2023
SRR6880642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13673855
Total Bases683.6 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC4163843.0451105412482438No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG3579752.617952289241037No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2675951.9569828698636924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2535701.8544148669120741Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1861951.3616862252817512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1850681.353444218912662Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1707541.2487626934759803Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1646931.2044372271023789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT1567171.146106931805259No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1542321.1279335637243484No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC1452231.0620487053577794Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA1445721.0572877948464423Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1399931.02380053028206Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1340310.9801990733410586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT1325850.9696241476891484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1260090.9215323696207105No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1097960.8029630268859805No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC1096140.8016320196462519No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1057180.7731396888441482Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1055380.7718233080576034No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG1011160.739484220068152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC954570.6980986707845008Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC853780.624388659964582No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT851440.6226773649420737No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA814020.595311271035125No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA797590.5832956397446075No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA778780.569539460525214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA754730.5519511505716567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT748130.5471244210209922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT711940.5206578539848492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG706240.5164893148274573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC683290.4997054597990106No Hit
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT658050.4812468758810153Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC656770.48031078287725004No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT649660.47511107877039793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT642760.4700649524219761Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC635000.46438988858664954No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG601710.440044157262162Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA596830.4364753026853071Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG576040.42127110460071426No Hit
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA568950.41608602694704605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG555580.40630824299365464No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG518160.378942149086706Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG505230.3694861471033589Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC501620.36684607230367733No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC477790.34941865333514216No Hit
TTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACT459700.3361890264303666Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCC445510.3258115578964381Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC442270.3234420724806574No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG438660.32080199768097584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC437800.3201730601940711No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG432520.3163116765535396No Hit
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA414470.3031113025551317Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC409180.29924260568800826No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA390960.28591790683753776No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT383200.2802428430022112No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG380560.2783121511819454No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT361730.2645413455093681No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG356140.2604532518444872TruSeq Adapter, Index 3 (100% over 37bp)
TTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAAC353210.2583104764530558Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA337950.24715049267379244No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC330340.24158512723734454No Hit
CCGTGGCGCAATGACGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT317510.23220225751991666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACAC305550.22345563851598546No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT296130.2165665790664008Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA288650.21109628557564783No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACAC286290.20937036409995574No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGC283350.2072202754819325No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTGA272130.19901483524580305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC271800.1987734987682698No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT258280.18888601641599972No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCAGATCGGAAGAGCACACGTCT254860.18638489292156454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA253320.1852586560264095No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTAGATCGGAAGAGCACACGTCT246370.1801759635450281Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA242610.17742619034646778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT241740.1767899396329711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA240680.176014737614228No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGAGATCGGAAGAGCACAC239380.17506401815727898No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCAC237330.17356480670593627No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCAGATCGGAAGAGCACACGTCTG236220.1727530385542336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA232300.16988625373020264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCAGATCGGAAGAGCACA232290.16987894050361074No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC229860.1681018264417752No Hit
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT224230.16398447987052664Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT220270.16108844214012802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCAGATCGGAAGAGCACACGTCTGAA218120.15951609842286613Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCACCCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG217210.1588505948030018Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT206260.15084261168485405No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC199570.14595006309486241No Hit
TTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACTC190990.1396753146789987Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGC185920.13596750879689745No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC182950.13379548049909845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC180150.13174777705336205Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA178940.13086287663574026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA175770.12854458380610295Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT172720.12631404969556864No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCAGATCGGAAGAGCACACGTCTGAAC170400.12461738112624421Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG166710.12191880051382731TruSeq Adapter, Index 3 (100% over 49bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC166160.12151657305127193Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC165510.12104121332279741No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG164510.12030989066360584No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC164230.1201051203190322Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC148980.10895244976636069No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGAGATCGGAAGAGC148900.10889394395362537No Hit
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA146180.1069047463206243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATACGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG145050.10607835171573782No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA145040.10607103848914588Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGCACTGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA144200.10545672745542498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC143070.1046303328505385No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGAGATCGGAAGAGCACACGT142780.10441824927937293No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG142460.10418422602843164No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC141810.10370886629995711No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGGAGATCGGAAGAG138070.10097371955458062No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)