FastQCFastQC Report
Wed 5 Jul 2023
SRR6880643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30454611
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG14874584.884179935839601Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC12322334.0461295007182985Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC4838281.5886855359932197No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA4621891.517632256081025Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG4529471.4872854557229447No Hit
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC3539701.1622870507195118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC2961390.9723946235924668No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC2906530.9543809310189515No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT2871730.9429540899406005No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT2689870.883238994581149Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA2525010.8291059767599723Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2504410.8223418122135923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2481840.8149307833877767Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2418960.7942836636462045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2370950.7785192199631117Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2324510.7632702975585536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG2012490.6608161897060514Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1885810.6192198613208357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1866140.6127610692515495Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1799450.5908629074263992TruSeq Adapter, Index 3 (100% over 49bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1746050.573328616806171Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG1641440.5389791384956452TruSeq Adapter, Index 3 (100% over 37bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1583970.5201084328412535No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1575100.517195901796283Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG1554760.5105171102004882TruSeq Adapter, Index 3 (100% over 38bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1526830.5013460851626047Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1477250.4850661202009771Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1429770.4694757059940775No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1265990.4156973142753325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1241570.4076788240703517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1239000.4068349452895655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1234490.4053540529544114Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA1208830.3969283994466388No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC1111360.3649233937021885No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG1090830.35818221418096585Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1080880.3549150570335638No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1079340.35440938648009657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1077160.35369356712518835Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1064490.3495332775716623Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT975550.320329161321417Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA962330.3159882751416526Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA945180.31035694397804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG916490.3009363672384454No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC911060.29915338600122No Hit
TCTCCCGGGGAGCCCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAG880680.28917788508282044Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA877030.2879793801996026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG871400.28613072746192686Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG862140.28309013699107827Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC839100.27552478013920456No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC835700.27440836463154955Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC815930.2679167368120381Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA812100.266659127578415Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG791730.2599704852575526No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC764330.2509734896958625No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC732120.24039709454834277No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG725950.2383711287594512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA722620.23727769827695386Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC708620.23268069324543336No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA687940.22589026009887303No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT674120.2213523594177578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT663200.2177666954931718Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT619720.20348971129527807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT609030.1999795695962099Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC607230.1993885260921573Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG589830.19367510555298179Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589070.19342555385127067No Hit
ACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT583270.1915210803382122Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA582100.19113690206057796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA570480.18732138788441594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG568790.18676646370561095No Hit
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT566700.1860801965259054Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC560750.18412646938750918Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT530760.17427902789498773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT518890.17038142434326284No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC516400.16956381416265667No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC502800.16509815213203674No Hit
CTTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG501990.16483218255521306Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC488980.1605602514509215Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA477860.1569089160258852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC468540.15384862410490155Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA468340.15378295260445124No Hit
TAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT456550.14991161765290648Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAATACCACTACTCTCATCAGATCGGAAGAGCACACGTCTGAACTCCAGT440550.14465789761688305Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC439170.14420476426377601Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG437270.14358088500949823TruSeq Adapter, Index 3 (100% over 37bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG434370.14262864825296898No Hit
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG425340.13966358000763826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC414010.13594328950712914No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG413340.13572328998062066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT408150.13401911454393556Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC403990.13265314733456945Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG403130.1323707598826332No Hit
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA402990.132324789832318Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC401650.13188479077930104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGTCGGGGTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCT400410.13147762747650923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC398770.13093912117281684Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG384340.12620092241532818Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA382670.12565256538656822Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGCGTCTCTCGGGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCC382240.1255113716606001Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT369390.12129197775666876No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA367220.12057944197678308No Hit
ATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGT359090.11790989548347867Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGAAC358990.11787705973325352Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC353880.11619915289674855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACA347180.11399915763166374No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAA344330.11306333875024704Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC338040.11099797006108533Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGAAC337930.11096185073583766Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA335660.11021647920572684Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA331190.1087487211706628Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC330750.10860424386967214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC329800.10829230424253325Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT325300.10681469548240166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCCGAGGCCTCTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA322970.10604962250215576Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGT318090.1044472378911686No Hit
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT317850.10436843209062825Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)