FastQCFastQC Report
Wed 5 Jul 2023
SRR6880644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31407489
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC13130994.180846803767089Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG8920122.84012516887294Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC6213291.9782829502861563No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC4782011.5225699832291593No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG4551811.4492753623188406No Hit
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4445321.415369436251335Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT4100071.3054434246558202No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA3599531.1460737915087704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA3554721.1318064936678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC3341291.0638513636031202No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA2889220.9199143554583431No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG2838300.9037016617278765No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC2817560.8970981411471639Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2721760.8665958618977785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT2668230.8495521561752357Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT2655540.8455117185585898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC2470330.7865417066611088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2444340.7782666102342661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG2383270.758822203201281No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2365540.7531770527723499Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC2362730.7522823617004212Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG2285170.7275876145335911Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2249860.7163450729856181Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC2062150.65657907258998No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2058610.6554519528765894Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG1932330.615244981857671Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1836880.5848541409980276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1781800.5673169224066273TruSeq Adapter, Index 7 (100% over 37bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1637260.5212960513971684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1620660.5160106877694043No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT1571280.5002883229538025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1528610.4867023912672548No Hit
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT1485080.47284263953734096Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1405020.4473519038723535No Hit
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT1302510.4147131914939141Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT1265730.4030026087090248Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1212640.38609899696215766Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1099490.350072557535561TruSeq Adapter, Index 7 (100% over 49bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG1087870.34637280299612616Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1072620.341517273157367Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1029280.3277180165533131Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT1002450.31917546799108965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT999740.3183126164590872TruSeq Adapter, Index 7 (100% over 38bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG982350.31277572046590546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCTGAACT942150.29997622541553703Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA925410.2946462864318761Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC887340.28252497358193773No Hit
TTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCC846890.26964587968175363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA844570.26890720235546367No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGT823610.2622336347869134No Hit
TTTCCTCCCGACCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA799730.25463035265251543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT797710.2539871939460044No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA781570.24884829220190124Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA775770.24700159888617648Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG761910.24258863865239275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC752820.23969442447309303No Hit
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTC734220.23377227004680318No Hit
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA732900.23335198811977614Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT729290.2322025807284371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG726490.231311073610501Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG719380.2290472823217418No Hit
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA713970.22732476321172956Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTG712800.22695224059459196Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG707330.2252106177606239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT674990.21491371054846187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC672280.2140508590164594No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC667320.21247161783611546Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC640670.20398638044575929No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG634190.20192317825853573No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC626110.19935054343249153No Hit
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG613850.19544701583752844Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC574490.18291497292254086Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTC546340.17395214243329035No Hit
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA541350.1723633493909685Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT540570.17211500097954344Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG527510.16795675706517002No Hit
TCTCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGT525200.16722126369287274Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTCTGAAC517480.16476325121056318Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCA515530.16414238018200056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG497040.1582552492496296No Hit
TTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACT494900.157573883095207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA493440.15710902581228317Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA490720.1562429903262881Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG484540.1542753067588434No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAA475520.1514033802574921Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG470740.14988145024901545Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACG456880.1454684900152317No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA455270.14495587342241845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA454220.14462155825319242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA447910.14261248328384354Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG445670.14189927758949467Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT437460.1392852513615463Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT429000.13659162628378219No Hit
GTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC424190.13506014441332767Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT409760.1304656988019641No Hit
TCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA386450.12304390204514598Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCT385940.12288152039152189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCT384030.12227338517892977Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA379950.12097433194993717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA379750.12091065287008458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC378540.12052539443697648No Hit
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGT378020.12035982882935978No Hit
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC371310.11822339570030575Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC361010.11494392308789793No Hit
ACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGT344870.10980502134379479Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT342770.10913639100534271Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA339700.10815891712960561No Hit
TATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT330790.10532201412217321Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC326310.10389560273347544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGTCTGAAC325800.10373322107985136Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG323250.10292131281173099Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC322280.10261246927444596Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC320520.10205209337174329No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT317450.10107461949600619Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGAGATCGGAAGAGCACACGTCTG316670.10082627108458113Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTGAACTC315260.10037733357162046Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)