FastQCFastQC Report
Wed 5 Jul 2023
SRR6880645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45111010
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC26568305.889537831230114No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG18551034.1123065078791186No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC10859752.407339139602505No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG8803801.9515856550318869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC6050831.3413200014807916No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG3150850.6984658512411936No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT2996600.6642724248470606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT2707860.6002658774432229No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA2655020.5885525506966037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2625190.5819399742989572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2548840.5650150595165127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2438700.5405997338565462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT2303110.5105427699357651TruSeq Adapter, Index 1 (100% over 35bp)
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT2302830.5104807008311275Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA2269460.503083393610562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2130540.47228825069534025No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2032100.4504665269077327No Hit
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC1733270.3842232749832026Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA1728200.3830993808385137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1669030.3699828489763364Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1517930.3364877000093769No Hit
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1470370.3259448192359249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1446680.3206933296328324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCGTGCATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCT1334830.29589893908382897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC1232540.27322376510745383No Hit
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC1224230.27138164275195786No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC1153190.2556338242038917No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1116330.24746286992909267No Hit
TTACAGTGAAACTGCGAATGGCTCATTAGATCGGAAGAGCACACGTCTGA1113980.24694193280088383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA1110160.2460951328733274No Hit
TTTGGAATGAGTCCACTTTAAATCCTTTAGATCGGAAGAGCACACGTCTG1100540.24396261577827677Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1092840.24225571540074142Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG1081620.23976851770776136No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT1081550.23975300043160194No Hit
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT1078160.239001520914739No Hit
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC1058750.23469880191110773Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG1051800.23315815806385182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG1035850.22962243585324293No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1020410.22619976808322403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTC984930.21833472582413918No Hit
TAGTGACGAAAAATAACAATACAGGACAGATCGGAAGAGCACACGTCTGA954140.21150934106773492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA927370.2055750913136283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA905400.20070488335330997No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTC900330.1995809892086211No Hit
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA886490.19651300203653166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA885080.19620043975960635Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA872270.19336077822243394Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTCT865650.19189328724850097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT851750.18881199955398917No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC839480.1860920427186179No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT838780.1859368699570238No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA835390.18518539044016083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG833060.18466888681942611TruSeq Adapter, Index 1 (100% over 49bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA815300.18073193218240957No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC813370.1803040987111572No Hit
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA802290.17784793557049597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTG764030.16936663577250877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC752900.16689938886316225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG750600.1663895355036387No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG744620.1650639167688775No Hit
ATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGA737970.16358977553373333Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG732030.16227302381392036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCT728670.16152819455826858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC719980.15960183556076443No Hit
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC706840.1566890211502691Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG696190.1543281784203014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT686390.1521557597579837No Hit
TATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAA684410.15171684251804604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACAC664970.1474074732532036No Hit
AAATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCT657980.14585796239099943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACAC640240.141925441261457No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT639300.14171706641017348No Hit
TCCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT626910.13897050852995754Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT622020.13788651595253576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA618010.13699759770397515Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCTG600250.13306064306695858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA599890.13298083993242446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC587420.13021654802231206No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG580460.1286736874213191No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT578200.1281727010767438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA574350.12731925088797613No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA564790.12520003431534785Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA538590.11939213952425361TruSeq Adapter, Index 1 (100% over 37bp)
TCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGT534960.11858745791770126No Hit
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA530670.11763647056450299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTAGATCGGAAGAGCACACGTCTG524220.11620666440410002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT524110.11618228011299238No Hit
TACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC520290.11533548018543588No Hit
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC506490.11227636002829465Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG506450.11226749301334642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC504870.11191724592289111No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC495410.10982019688763342Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTAGATCGGAAGAGCACACGTCT494320.109578570730294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG492510.10917733830388635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG486320.1078051677406469No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA484220.10733964945586455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCACACGTCTGAA481230.10667684008848395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA472880.10482585071803978No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT471190.10445121933647684No Hit
AGGGCGCACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGT470120.10421402668661153No Hit
TCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACACG468460.10384604556625976No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA468210.10379062672283329Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAGCGTTGGATTGTTCACCCACTAATAGATCGGAAGAGCACACGTCTGA464920.10306131474334093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCT463260.10269333362298916Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAATTCACCAAGCGTTGGATTGTTCAGATCGGAAGAGCACACGTCTGA459530.1018664844790662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCGAAAGCATTTGCCAAGAATGTTTTCAGATCGGAAGAGCACACGTC455220.10091106361839383No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC454110.10066500395358027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA452280.10025933801969852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)