FastQCFastQC Report
Wed 5 Jul 2023
SRR6880646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39620305
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG10527412.6570744470543577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC6635801.6748482880179747No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGC5555591.4022077820955694No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCA5454711.3767460901676551No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG5028241.2691068380215649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3928540.99154713725702No Hit
TTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC3398730.857825299426645Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG3106770.7841358111705601Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAG2912400.7350776325422028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2494050.6294878345838074Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2455370.619725163650305Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA2378290.6002704926173587Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2067720.5218839178547464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC1884950.47575353092309614Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG1532560.38681176230218317No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1477450.3729022277844656No Hit
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG1435750.36237732142647566Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1427010.36017138182050845TruSeq Adapter, Index 5 (100% over 35bp)
ATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1408030.35538090885468954TruSeq Adapter, Index 5 (100% over 35bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1377200.3475995452331828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAG1367900.34525226395909875No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1362690.34393728165393983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGA1332800.33639317011820075No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG1293750.32653711272540686No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACG1272400.32114846162845034No Hit
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1264250.31909143556567776Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA1203050.30364481040718894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC1035450.2613432683064908No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGT1014940.25616662971170967No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGT979720.24727724837050094No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTC943270.23807742015110686No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG889320.22446066480305993No Hit
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA838790.21170710321386974Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG830090.2095112594413395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC807380.20377935000752773Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGT773510.19523070304481502No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG772340.1949353999167851TruSeq Adapter, Index 5 (100% over 37bp)
TCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA768410.19394348428160763TruSeq Adapter, Index 5 (100% over 36bp)
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC742380.1873736206725314No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA742360.18736857275581295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA719210.18152560915419505No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT712810.17991027580428773Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAAC697300.17599561638912167Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTG687990.17364581115667838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCAC673440.16997345174399844No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT673230.16992044861845462No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA660400.16668221004356226No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC649370.16389828397333134No Hit
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG627660.1584187703754426Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCA614330.15505433388258874No Hit
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT607490.1533279463648753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC591760.14935775986580618Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG559590.14123818582416264No Hit
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG551370.13916349205287543Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAAC538140.13582429514361385Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC530960.13401209304168657No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC511190.12902222736548846No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC499740.1261322950441699Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA478400.12074616790557266Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGCGGGGGGCCCGGACACAGATCGGAAGAGCACACGTCTGAACTCC476230.1201984689416197Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC468450.11823482933813861Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTG460650.11626614181793905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAG456750.11528179805783928No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCAGATCGGAAGAGC447850.1130354751181244No Hit
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC442520.11169020531265471Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAACT423380.1068593490130881Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGAAC401500.10133692812309245Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCTCAACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGT397420.10030715311252653No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)