FastQCFastQC Report
Tue 4 Jul 2023
SRR6880647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21014740
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC17031038.104325820828619No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG6950683.3075260507624646No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA5468882.602401933119325No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC5204422.4765569309922464No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC4923392.342826987152827No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT4078411.9407377869057625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT3716471.768506296056958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3506031.6683670604537577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2830841.3470735302934986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACAC2759531.3131402053986867No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG2671021.2710221492152651No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC2605081.2396441735657924No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT1801440.8572268797996073No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACG1791760.8526205891674129No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC1626680.7740662030555696No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTAGATCGGAAGAGCACA1274910.606674172509391No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA1209050.5753342653775397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACAC1054370.5017287865564837No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1044780.4971653230066135No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT962620.45806895540939363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC916370.43606059365949806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC862170.4102691729709718No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACG854330.4065384582440706No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA846700.4029076733759257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA838970.3992293028607539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT824020.3921152486302471Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT785920.3739851171130359No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC764540.3638113057787058No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA738090.35122490214011687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACAC710400.33804843647839566No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACG690710.3286788225788185No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT679250.3232255074295471Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT677790.3225307569829558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGCCACGATCACAAGAAAGACGTGGTCCTGAGATCGGAAGAGCACACG652600.31054393249690454No Hit
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT641650.30533330414746984No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG632390.30092687323278805No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA630610.300079848715711No Hit
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT619030.2945694307900074No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC595860.2835438363738976No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACA551590.2624776704351327No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG550840.26212077808243167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT537350.2557014742985162No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTAAGATCGGAAGAGCACACGTCTGA536010.255063826628357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACA487880.23216085471435763No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT475350.22619837314189944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGC466940.22219642022694547No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGA460540.2191509388172302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG455110.2165670381836749No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC452300.21522988150222178No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACA414420.19720443840846946No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC406590.19347848224627096No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC403880.19218891121184464No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT399110.1899190758486662No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACG398010.18939563373137142No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG392580.1868117330978161Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG379820.18073980453719626No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC376860.17933126938520297Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT375160.17852231338574734No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA367160.17471546162360324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGAGATCGGAAGAGCACA354910.1688862198628201No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG353500.1682152622397422No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG337590.16064438579777812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG335150.15948329601032418No Hit
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG329230.15666622570633754No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG328690.15640926321239282No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC318300.1514651144863082No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC316810.15075608834560886No Hit
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG311310.14813887775913478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC311280.14812460206502673Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA308990.147034890748113No Hit
TCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACG307740.146440070160278No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT297600.14161488555176033No Hit
TAGGCGAGTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGT293550.1396876668471749No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC288410.13724176458999732No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGAGATCGGAAGAGCA283890.1350908933443859No Hit
AGGCGAGTCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTC280450.13345394708666394No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG273730.1302561916064629Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT268200.12762470532588077No Hit
CGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTC263800.12553093685670152No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG260730.12407005749297874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCACGCGTCTCCCGACGAAGCCGAGCGCAGATCGGAAGAGCACACG260180.12380833643433134No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC258720.12311358598774004No Hit
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCAC257760.12265676377628273No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT256260.12194297907088072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT248810.11839784836738404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG241380.1148622347932927No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG240810.11459099660523994No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC230840.10984670759666786No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTAGATCGGAAGAGCACACGT227670.10833824258591827No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTAGATCGGAAGAGCACACGT227060.10804797013905477No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC226840.1079432817155958No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACAC225200.10716287710435628No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA223240.10623019842263097No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACAGATCGGAAGAGCACACGTCTG222610.10593040884636212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTG222490.10587330606992996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT222130.10570199774063349No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC221410.10535938108204052No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT219730.10455994221199025Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTG219470.10443621952972058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACA216470.10300865011891654No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGA216130.10284685891902541Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC214640.10213783277832607No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT214430.10203790291956978No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCAGATCGGAAGAGCACACGTCT214250.10195224875492155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)