FastQCFastQC Report
Wed 5 Jul 2023
SRR6880648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29544760
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC20872567.064724844608655No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG10975633.7149159444855875No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC7056092.3882712196680562No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG7008002.3719942216487797Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA6060162.0511792954148214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT4595411.5554061024696089No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG4486381.5185027734190428No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG4221011.428683123504811TruSeq Adapter, Index 4 (100% over 49bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3352341.134664827197784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG3263531.1046053513381053Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT2929740.9916276185692489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2660500.9004980917089866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG2261470.7654386090799181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC2245230.759941864479522No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA2207780.7472661818880911Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2113950.7155075891630192No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA1921820.6504774450697857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1793960.6072007354265189No Hit
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC1787660.6050683776073997Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG1774000.6004448843043572No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1577530.5339457826023972Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA1565900.5300093823744041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC1450890.49108200574315036No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA1412090.4779493893333369Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1319500.4466104987821868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA1292660.4375259775337488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1262920.4274598947495258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1258250.4258792422074168No Hit
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC1212680.410455187315788Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC1135950.3844844229568966No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT1135690.384396420888171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1133810.3837600982373862No Hit
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC1093770.3702077796536509Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT1080300.36564859555467705No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1003520.33966090772103075No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACA977520.3308607008484753No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT953820.322838973814646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT938330.31759608133557354No Hit
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA925520.3132602871033645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG922600.3122719561776775No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG917650.310596532176941Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA880880.2981510088421771No Hit
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC880070.2978768485511475TruSeq Adapter, Index 4 (100% over 38bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA877150.2968885176254605Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC831580.28146446273383163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC826920.2798871948866737No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA824600.27910194565804564No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT816990.2765261928003477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC813810.2754498598059351Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC806390.27293841615230585Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAA800290.27087375223220633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC758400.2566952650825392No Hit
ACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA756830.2561638679752349Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG715720.2422493870317444No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC712610.24119674690198872Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT692710.23446120394953282Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA667850.22604685230138952Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG661590.22392803326207422No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC650380.22013379022202245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG647630.21920299911050217No Hit
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT635180.21498905389652853No Hit
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG608590.2059891500218651No Hit
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA596570.20192074669078375Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG587020.19868836301259515No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC586510.19851574357009502No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA572450.1937568624690131No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT564880.1911946483911191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT556490.1883548893272445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC550120.18619883864346842No Hit
AACGGGACGGGAGCGGCCGCGGGTGAGATCGGAAGAGCACACGTCTGAAC544960.18445233604876127Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG519310.17577059349949026No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTCTGAAC517290.17508688511939172Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA515130.17435579100997944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCA513420.17377700817336136Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT488510.1653457330504631No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCAC482550.1633284548596773No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG475920.1610844021071757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT474710.16067485401810677Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCAC471810.15969329248232175No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG462510.15654552617790768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC459920.15566889018560315No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG455020.15401038965962155No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC441760.1495222841546183No Hit
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA441310.14936997288182405Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTC439110.14862533999260782Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT429170.14526095321133087No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT426940.1445061662372617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAG426460.14434370087961454No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC422970.1431624423417215Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGCCACGATCACAAGAAAGACGAGATCGGAAGAGCACACGTCTGAACT409850.13872172256603202Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC407160.13781123962421762Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCAC402550.13625089525181455No Hit
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC396730.13428100279034252No Hit
TCACCACCGGCCCGTCTCGCCCGAGATCGGAAGAGCACACGTCTGAACTC396060.13405422822862667Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCAGATCGGAAGAGCACACGTCT394510.1335296005112243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG389280.1317594050518603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC382370.12942058084073116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCAGATCGGAAGAGCACA370470.12539279384906157No Hit
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGAGATCGGAAGAGC366720.12412353324244298No Hit
ACGAGGCGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACG364650.12342290138758953No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAAAGATCGGAAGAGCACAC361370.12231272144366716No Hit
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC346100.11714429225351637TruSeq Adapter, Index 4 (100% over 37bp)
TAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA341450.11557040910130933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACACGTCTGA338350.11452115366650466Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA333370.1128355755809152Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC328750.11127184651356112No Hit
TCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACACGTCTGAAC326110.11037828704650164Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC318430.10777884132414682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG314910.10658742870140085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG311600.10546709467262552No Hit
ACCCCCCGTCCTTTCCGCCCGGGCCCGCCCAGATCGGAAGAGCACACGTC307470.10406921565786961No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCGTCAGATCGGAAGAGCACACGTCTG304010.10289811120482956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT296930.1005017471795337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA296350.10030543487237668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)