FastQCFastQC Report
Wed 5 Jul 2023
SRR6880649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34851694
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG13838803.9707682501745825No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC12874313.694027039259555No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG12467313.577246489080273Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA12125683.4792225594543558Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA9372672.6893011283755675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC7958512.2835360599688497No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4894991.4045199639363297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA3977211.1411812579325413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3339810.9582920130080333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT2893760.8303068424737116No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT2850130.817788082266532Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC2802650.8041646411792782No Hit
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2735890.7850091877886911Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA2681720.7694661843409965No Hit
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC2315650.664429683102348Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2130070.6111811953817797Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1975700.5668877960422813Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA1943440.557631431057555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT1900900.5454254246579807No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1786840.5126981775979096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA1763250.5059294965690907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1748220.5016169371853202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG1527860.4383890206312496No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1504940.4318125827685736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1467920.4211904305139371Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1436680.4122267342299057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC1358690.38984905583068646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTCAACCCGGCGGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1327540.38091118325553985Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1325330.38027706773736736Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1321300.3791207394395234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG1289370.3699590613873747No Hit
TTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGTCT1211900.3477305866394902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1138760.3267445192190658Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC1129990.32422814225328617No Hit
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA1126810.32331570453935465Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1084940.3113019413059233Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTG1069270.3068057466589716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1046700.30032973433084775No Hit
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC1043660.2994574668307371Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT1043160.29931400178137685No Hit
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT994330.28530320506085016No Hit
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA965390.2769994480038761Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA944450.2709911317366668Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG918260.26347643245117436No Hit
CCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG916120.26286240203991235No Hit
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG914970.26253243242638363Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG910540.2612613320890514Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT908340.2606300858718661Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGT869730.2495517147602639No Hit
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA857630.24607986056574466Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG852820.2446997267908986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC800980.2298252704732229No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC793000.22753556828543253No Hit
TCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC784740.22516552567000042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA738780.21197821833280184Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG737580.21163390221433714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGAGATCGGAAGAGCAC735100.21092231556951005No Hit
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC731390.20985780490325665No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGAGATCGGAAGAGCACACGTCT727520.20874738542120794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACTC720450.20671878962325332Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA714530.2050201634388274Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC707880.20311207828233543Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT692820.19879091099560325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGGGGCGGCGGGTCCAGATCGGAAGAGCACACGTCTGAACTCCAGT691330.19836338514850957Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA667460.19151436369204894Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC651520.18694069791844264No Hit
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA647230.18570976779493129Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT646400.18547161581299318No Hit
TCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACGTCTGAACTCCAGT643520.18464525712867788Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTCCCCCGCCGGGTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC631890.18130826008055734Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA627700.18010602296691805Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACG621310.17827253963609346No Hit
CCCCCCCCGGGCCGCGGTTTTCCGAGATCGGAAGAGCACACGTCTGAACT607460.1742985577688132Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG605740.17380503799901376No Hit
ACTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG599380.17198016257115079No Hit
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC596340.17110789507104016Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG587280.16850830837663158No Hit
TGGGCCCAAGTCCTTCTGATCGAGGCCCAAGATCGGAAGAGCACACGTCT584820.1678024603337789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC583700.1674810986232118No Hit
GCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA582000.1669933174553868Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG575750.16520000433838308Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT574050.16471222317055806No Hit
TTCCGCCCGGAGGATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACAC558320.1601988127176831No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG558000.16010699508609252Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTC550880.15806405278320187No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC550820.15804683697727864No Hit
TTGGGATCCCGAGGCCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT547330.15704545093274377Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC541410.15534682474831785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG526960.1512006848218052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA519640.14910035649917044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCCCGTCCTTTCCGCCCGGGAGATCGGAAGAGCACACGTCTGAACTC505750.1451148974279414Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCACA505430.1450230797963508No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA498550.1430490007171531Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGAACT495890.1422857666545563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC488760.14023995505067846Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG476690.13677670875912085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT457620.1313049517765191Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG452310.12978135295231272No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC446500.12811428907874609No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT440050.12626358994199824No Hit
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG439590.12613160209658675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGCCACGATCACAAGAAAGACGAGATCGGAAGAGCACACGTCTGAACT436480.1252392494895657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC435230.12488058686616495Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT434900.12478589993358716No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGA434420.12464817348620126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC418820.12017206394615998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA418010.11993965056619629Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA414720.11899565054140553No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT414410.11890670221080214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCC413700.11870298184071054Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG405940.11647640427463869TruSeq Adapter, Index 8 (100% over 38bp)
TCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCTGAACT401260.11513357141262631Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT393490.11290412454556728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG382300.10969337674088381Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGGCGCAATGAAGGTGAAGGGCCCAGATCGGAAGAGCACACGTCTGAAC378860.10870633720128496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG373640.10720856208596345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG372990.10702205752179507TruSeq Adapter, Index 8 (100% over 49bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC370020.10616987512859491TruSeq Adapter, Index 5 (100% over 35bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC363890.10441099362343764No Hit
TGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCTGA360510.10344116988976204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCAGATCGGAAGAGCACACGTCTG356260.10222171697019951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACG353850.10153021543228287No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC353100.10131501785824243No Hit
TCTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCA350980.10070672604895474Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC350810.10065794793217225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)