FastQCFastQC Report
Tue 4 Jul 2023
SRR6880653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36189104
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC13005253.5936921787287135No Hit
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC7297962.016618040612445No Hit
ATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG7128951.9699161382940014Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGT6383531.7639370126433636No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACG6103911.6866706619760468No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA5981561.6528621432572634No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC5779591.59705252719161No Hit
TTTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGA5207401.4389414007044772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA5167521.4279215091923803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT4754301.3137379693070046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCT4465921.2340510005442522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCA3833191.0592110818770202Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA3733991.0317995162300786Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT3562860.9845118022264381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCC3366980.9303850131243925Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCT3311490.9150516685906342Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACG3197820.8836416618659584No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACAC3163400.8741305117695095No Hit
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT3126870.8640363132505299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTC3024730.8358123483797775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGAACT2848350.7870739214764753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGA2660270.7351024772539271Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAAC2570180.7102082438957317Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGCGTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACT2547440.7039245845931968Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTC2403620.6641833409304635Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG2390570.6605772831513044No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA1954650.5401211370140582No Hit
TCGGGTCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTG1827560.5050028317915801Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT1806410.49915853125294285TruSeq Adapter, Index 8 (100% over 37bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG1790820.494850604756614Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCAC1726240.47700545445944176No Hit
TTCCTCCCGACCCCTCCACCAGATCGGAAGAGCACACGTCTGAACTCCAG1692540.4676932592749464Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACGT1638270.4526970327864431No Hit
CCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTCT1465880.40506114768688384Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACAC1411210.3899543906917397No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACGTCT1406560.38866947355203935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1352540.3737423286301866TruSeq Adapter, Index 8 (100% over 49bp)
TTTCCTCCCGACCCCTCCACCCGCGCGTAGATCGGAAGAGCACACGTCTG1296650.3582984535897877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCCTCCCGACCCCTCCACCCGCGCAGATCGGAAGAGCACACGTCTGAA1294880.3578093560979017Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGT1288660.3560906067196358No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACA1225210.338557705103724No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAGATCGGAAGAGCACACGTC1214390.33556785489908786No Hit
TTTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAG1209620.3342497786073952Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCAGATCGGAAGAGCACACGTCTG1165450.32204444741157445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAGATCGGAAGAGCACACGTCTGA1087740.3005711332339148Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG1004930.2776885550965838TruSeq Adapter, Index 8 (100% over 38bp)
TTCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGT1001530.2767490457901362No Hit
TGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGAA993480.2745246193439882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCTCCCGACCCCTCCACCCAGATCGGAAGAGCACACGTCTGAACTCCA992550.27426763591604814Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCTCCCGACCCCTCCACCCGCGCGAGATCGGAAGAGCACACGTCTGAA989990.27356024067354634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCCTCCCGACCCCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC979030.2705317047915859Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCAGATCGGAAGAGCACACGTC966510.2670720999337259No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACAC953620.2635102543572231No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG937660.25910008714225147No Hit
TTCCTCCCGACCCCTCCACCCGAGATCGGAAGAGCACACGTCTGAACTCC848670.23450981267731855Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCAGATCGGAAGAGCACACG839130.23187366009393326No Hit
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCAGATCGGAAGAGCACAC834500.2305942694795649No Hit
TTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACG821930.2271208483083748No Hit
ACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTGAACTCCAG812340.22447087941165939Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGGCCCGAGATCGGAAGAGCACACGTCTGA789650.21820103642245467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTCT767960.2122075196998522Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGTAGATCGGAAGAGCACA729530.2015883012743283No Hit
AAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC717660.19830830848975978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA716480.19798224349516913Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGGGTCGGCGCGCGGCGCGGAGATCGGAAGAGCACACGTCTGAACTC708820.19586558429299603Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCAGATCGGAAGAGCACACGTC689360.19048827514491654No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGT685710.18947968427181838No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC684600.1891729621158899Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGTC658020.18182820994960253No Hit
TCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAAC652460.18029183590729408Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGAGATCGGAAGAGCACACGT635460.17559428937505608No Hit
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACG633310.17500018790186128No Hit
TTGGGATCCCGAGGCCTCTCCAGATCGGAAGAGCACACGTCTGAACTCCA617610.1706618655162062Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAGATCGGAAGAGCACACG614980.16993512743504233No Hit
TCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAG572930.15831560792441834Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCAACCCCGCGGGGGTTCCGGAGCGGGAGGAGATCGGAAGAGCACACGT572300.15814152237645895No Hit
TGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCTGA561820.15524562310246753Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA554830.15331410249891791Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTCTCGGGGTCGGGGGTGAGATCGGAAGAGCACACGTCTGAACTCCAG542880.1500120036130212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCCCGTCCTTTCCGCCCGGAGATCGGAAGAGCACACGTCTGAACTCC538440.1487851149893073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGT533110.14731229598831738No Hit
TCCCCCGCCTCCCCTCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAG522620.14441363345165992Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCTCGCTTCTGGCGCCAAGCGTCCGAGATCGGAAGAGCACACGTCTGAA518930.14339398952789767Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCTGA497410.13744744827061758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCCTCCCGACCCCTCCACCCGCAGATCGGAAGAGCACACGTCTGAACT497290.1374142891186253Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGAGATCGGAAGAGCACACGTCTGAACT480610.13280516699170003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCTCGGCGCCCCCTCGATGCTCTAGATCGGAAGAGCACACGTCTGAA475730.13145669481068115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCTCGGCGCCCCCTCGATGCTCTTAAGATCGGAAGAGCACACGTCTG474980.13124945011072944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACAGTGAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTG474110.13100904625878554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTCTG472340.13051994876689957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCA464840.12844750176738282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGATCCCGAGGCCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAG463630.1281131469847941Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGTC461910.12763786580623826No Hit
CCTCTCGGCGCCCCCTCGATGCTCTTAGAGATCGGAAGAGCACACGTCTG459170.12688073183574813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCTCCCGACCCCTCCACAGATCGGAAGAGCACACGTCTGAACTCCAGT455220.12578924308266928Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCCGGCGGATCTTTCCCGCTCCCCAGATCGGAAGAGCACACGTCTGAA453470.1253056721161154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGTCGGGGGTCGGCGCGCGGCGCGGGAGATCGGAAGAGCACACGTCT450180.12439655869899405Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGCGGGGGGCCCGGACACTAGATCGGAAGAGCACACGTCTGAACTC440060.12160013688097943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTGAA439580.12146750027301036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTAGATCGGAAGAGCACACG423770.117098781998029No Hit
TTGGGGCGGGGGGCCCGGACACTCGAGATCGGAAGAGCACACGTCTGAAC419630.11595479125429578Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACACGTC417780.11544358766108163No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC411760.11378010353613617No Hit
ATCCCGAGGCCTCTCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC405310.11199779911655176Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG399240.1103204986782762No Hit
GTTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTC399060.1102707599502878No Hit
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACA389640.10766776651889474No Hit
AAAACTGCGAATGGCTCATTAAGATCGGAAGAGCACACGTCTGAACTCCA388750.10742183614161877Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC388220.10727538322031957No Hit
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT387810.10716208945101267Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGAGATCGGAAGAGCACAC386160.10670615111111896No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC383400.10594349061529681Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC381470.10541018092075449No Hit
TCTCTCTCGGGGTCGGGGGTGCGTGGCGGGGAGATCGGAAGAGCACACGT363480.1004390713845803No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)