FastQCFastQC Report
Fri 7 Jul 2023
SRR6880655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74874874
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC63770828.516985284008625No Hit
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG43951265.86996113008484Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA19955932.6652372062756324Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG12854021.7167334398452545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG9925211.3255728483763458No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA9018081.2044200568537853Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG7431980.9925866452877102Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGTCTGAACTCCA7207110.9625538735464182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC7148800.9547662143645143No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT5543310.7403431490248651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG5517440.7368880513909112No Hit
ATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA5174020.6910221979138156Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG4953800.6616104622760367No Hit
TATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAACTCC4923090.6575089528698239Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG4519820.6036497637378329Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA4388600.5861245255651449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC4283640.5721064719254152No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA4089320.546153840606129Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAACTC4002580.5345691800429607Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT3531410.4716415282381644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACGTCTGAACTC3422410.45708390774721036Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC3394790.45339508684849344TruSeq Adapter, Index 5 (100% over 35bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA3319420.44332895972552827Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC3221060.43019237668433336Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC3205710.42814228976198343No Hit
ACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACACGTCTGAACT3055610.4080955114528807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2966740.39622637628745794Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2959180.3952166917836817Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAACTCCA2784240.37185237867645693Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2777600.3709655658318704Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACA2731860.36485670747172144No Hit
ATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC2712200.362230993537298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG2711490.3621361686698798Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT2641250.35275518460304855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2606280.34808472599232687TruSeq Adapter, Index 5 (100% over 49bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA2556820.3414790387493674No Hit
AACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2518500.3363611670318136Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA2485300.3319271028088809Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCTGAACTCCAG2463150.3289688340577374Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG2363830.3157040371113012Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAACTC2307610.30819551028559994Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA2307280.3081514367556732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT2296880.3067624527822244Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA2254940.3011611077969894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA2124570.28374939235290064Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA2082700.2781573962982562No Hit
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA2069890.2764465420001909No Hit
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC1976790.264012464314798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGTCTGAA1908240.2548571901436522Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1843120.24616001357144188Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT1688070.22545213231343803Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCCGTCCCCGCCCCTTGAGATCGGAAGAGCACACGTCTGAACTCCA1666800.22261139297543256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1662250.22200371248704875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC1579600.21096529658266938Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT1575050.21035761609428552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG1519250.2029051828521274Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGCCCGTCACGTTGAACGCACGTTCGTGAGATCGGAAGAGCACACGTCT1481470.197859431456272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGGGCGGCGGGTCCAACCCCGCGGGGGAGATCGGAAGAGCACACGTC1468620.19614323491215493No Hit
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG1421650.18987010248591535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTC1420800.1897565797573162No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG1336890.17854988310230746No Hit
AAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTGAACTCCAG1301410.17381131085442628Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1300500.17368977475674952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAACT1289180.17217791912411098Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA1264840.16892716240163555Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGT1235280.16497924256940988Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGA1223620.16342197784533166Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAAAGATCGGAAGAGCACACGTCTGAAC1211300.16177656606139998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1208760.16143733343711536No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG1159650.15487839084710847No Hit
TGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGT1133790.15142462877466745Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1124130.15013447635317556Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCA1110420.1483034215189464Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATACATGCCGACGGGCGCTGACCCCCCTAGATCGGAAGAGCACACGTCT1106580.14779056589798067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC1089840.14555483592533325Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCCGGCGGCGCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1065900.14235750166337507Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCT1053630.14071876768700806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1051220.14039689736239155No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCAGATCGGAAGAGCAC1028830.1374065751349378No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT1015440.13561825826912244No Hit
AAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1013730.13538987725041113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1011990.13515748954716103Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCCCCGAATCCGGAGTGAGATCGGAAGAGCACACGTCTGAACTCCAG1010570.13496783981232477Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG1007520.13456049355088062No Hit
ACCACCGGCCCGTCTCGCCCGCCGAGATCGGAAGAGCACACGTCTGAACT979460.13081290794559466Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGAAGGTGAAGGGCCCCAGATCGGAAGAGCACACGTCTGAACTCCAG978590.13069671409396963Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCAC969400.12946933306358552No Hit
GGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC955340.12759153357640377Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCGGTCGGGCTGGGGCGCGAAGCGGGGAGATCGGAAGAGCACACGTCTG948100.1266245870410413Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAACTC915760.12230538110822063Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGGGTCGGCGGCGATGTCGGAGATCGGAAGAGCACACGTCTGAACTCC908860.12138384366429786Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC906260.12103659767093565No Hit
TCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGAGATCGGAAGAGCA883390.11798216849086118No Hit
ACGTTGAACGCACGTTCGTGTGGAACCTAGATCGGAAGAGCACACGTCTG871150.11634744119903292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGAGATCGGAAGAGCACAC865280.11556346659094212No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAGATCGGAAGAGCACACG835250.11155277536760862No Hit
TATGGTTCCTTTGGTCGCTCGCTCAGATCGGAAGAGCACACGTCTGAACT833360.11130035424166457Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATACATGCCGACGGGCGCTGACCCCCCAGATCGGAAGAGCACACGTCTG827790.11055644647896168Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC825780.11028799861486245Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAGAGCACACGTCTGA812450.10850769511812468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG808270.10794943040571929TruSeq Adapter, Index 5 (100% over 37bp)
TACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCA802470.10717480472821898Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACAC785750.1049417458785974No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT771450.10303189291510527No Hit
AAAAACCAACCCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC768600.10265125788391978Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC765380.10222120707675583No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCAGATCGGAAGAGCACA759920.10149199049069518No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA750320.10020985143828087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)