FastQCFastQC Report
Fri 7 Jul 2023
SRR6880656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6880656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43936947
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACACG10883172.4769973207287252No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC8678051.9751144748405027No Hit
ACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCACACGTCTGA8541791.9441018512278516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCACACGTCTGAA7099041.6157335647376683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCT6456131.4694079677406808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCTG5054591.150419031162998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTG4630501.0538966214470933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAATCCCGCCCAGGCGGAACGATACGAGATCGGAAGAGCACACGTCTGA3714070.8453181783431607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACAC3644550.8294955040913518No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG3023320.6881042508483804Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTCCCCGGCGCCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2926660.6661045429487853Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTC2886170.6568890642310673No Hit
ACCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCAC2827400.6435130779569186No Hit
ACCTGCGGAAGGATCATTAAGATCGGAAGAGCACACGTCTGAACTCCAGT2807990.6390953836642314Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGGCGGCGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA2165870.4929495897837417TruSeq Adapter, Index 7 (100% over 35bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACG1824720.4153042313112925No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCT1741560.39637710831387535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGT1722080.39194348209947316No Hit
ACCTGCGGAAGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1718970.39123564957756396Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACTC1663850.37869039922141157Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCCGTGGATCGCCTCAGCTGAGATCGGAAGAGCACACGTCTGAACTCC1586090.3609923101848656Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA1569190.35714588908510186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAACT1349960.3072493862625458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCTTGGTGGTAGTAGCAAATATTCAGATCGGAAGAGCACACGTCTGA1244150.2831671485959186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTTGAACGCACGTTCGTGTGGAACCTAGATCGGAAGAGCACACGTCTG1231580.2803062306536683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCA1206220.2745343230152063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1162360.2645518360663521No Hit
AACCATCCTCTGCTACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1130010.25718901224520674Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGA1108720.25234343205503107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1104780.2514466924613583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACACGTCTGAAC1021630.23252184545275756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACA1018600.23183222084137983No Hit
AAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACGTCTGAACTCCA973880.22165399885431275Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTCTG971750.22116921323641353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG966570.21999025102950373No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAA950730.2163850847442814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACACGTCTGAA948140.21579560364082648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC910820.2073016133779163Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAAGCGAATGATTAGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTC906710.20636618197436432No Hit
AGTTATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAAC896150.20396273778421609Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT881250.20057151444773802No Hit
ATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA866720.19726450269746781Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT856930.19503630964618457No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGCAGATCGGAAGAGCACAC837530.19062089134231378No Hit
TCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGAA829210.1887272686470455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTTCCTTTGGTCGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCA820180.1866720507458108Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACT819080.1864216919760037Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGGTCTTGGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC798630.18176729484640797Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG796980.18139175669169733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG795420.18103670243633452Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGA777020.17684888301410656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTATCAGATCAAAACCAACAGATCGGAAGAGCACACGTCTGAACTCCA765740.1742815676291755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTTATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAA760010.17297742603736213Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCACCGCCCGTCCCCGCCCCTTGCCAGATCGGAAGAGCACACGTCTGAAC742090.16889885407832275Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGAA741500.16876457073815346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCCGCGCGTGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCAC724140.16481345415283405No Hit
AATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTGAAC715840.16292438343519863Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCT705150.16049135139043685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG700170.1593579089598556TruSeq Adapter, Index 7 (100% over 49bp)
ATGCTAACTAGTTACGCGACCCCCGAGATCGGAAGAGCACACGTCTGAAC694940.15816756680886362Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACAGATCGGAAGAGCACA688110.15661306644724313No Hit
AAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGTCT687890.15656299469328172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACG673180.1532150151443158No Hit
ATTTATCAGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTC672380.15303293603900153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCGAGATCGGAAGAGCACACGTCTG662300.15073873931204185Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCCGGCGGATCTTTCCCGCTCCCCGAGATCGGAAGAGCACACGTCTGA658780.14993759124865913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACGTCT656550.14943004574259564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTCGATGGTAGTCGCCGTGCCTACCAGATCGGAAGAGCACACGTCT652450.14849689032786004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAG651350.14824653155805295Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC642900.14632332100817108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGTACACGGAGTCGACCCGCAACGCGAAGATCGGAAGAGCACACGTCTG639020.14544023734739694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAG637080.14499869551700986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGA627010.14270677477886662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTC625260.1423084767359917Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGGAGATCGGAAGAGCACACG619290.14094971141258403No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCGAGATCGGAAGAGCACACG607550.1382777005420973No Hit
ATAACCTAGACTTACAAGTCAAAGTAAGATCGGAAGAGCACACGTCTGAA606250.13798182199596162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA605940.13791126634265233Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGTTCCTTTGGTCGCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC599120.1363590419698483Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT593090.13498662071354206Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCAGATCAAAACCAACCCGAGATCGGAAGAGCACACGTCTGAACTCCAG586260.1334321203519216Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGATCCTTCTGACCTTTTGGGTTTTAGATCGGAAGAGCACACGTCTGAA576340.13117433944602477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGAAAGTCAGCCCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA555600.12645393864075263Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATGCTTGTCTCAAAGATTAAGCCAGATCGGAAGAGCACACGTCTGAAC552650.1257825219399063Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCAGATCAAAACCAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC550820.12536601598649993Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCCGTCACGTTGAACGCACGTTCGAGATCGGAAGAGCACACGTCTGA546370.12435320096318937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTACTTGGATAACTGTGGTAATTCTAGATCGGAAGAGCACACGTCTGA538070.12246413024555394Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGATCGGAAGAGCACACG537960.12243909436857321No Hit
AAGCCATGCATGTCTAAGTACGCACGGAGATCGGAAGAGCACACGTCTGA528580.12030421685876355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTATTCCCCATGAACGAGGAATTCCCAGTAGATCGGAAGAGCACACGT526820.11990364282707218No Hit
TTGAACGCACGTTCGTGTGGAACCTGAGATCGGAAGAGCACACGTCTGAA525800.11967149196779649Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAG520190.11839466224178026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACACGT516950.11765724186525749No Hit
TAATGAAGGTGAAGGGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA510690.11623247286617341Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCAAAACCAACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC510260.11613460534706702Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCAGATCGGAAGAGCACACGTC493290.11227225232558831No Hit
AGTTATGGTTCCTTTGGTCGCTCGAGATCGGAAGAGCACACGTCTGAACT488090.11108873814104563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC487310.11091121101336422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGAGATCGGAAGAGCACACGT486780.11079058360609353No Hit
ACGTTGAACGCACGTTCGTGTGGAAAGATCGGAAGAGCACACGTCTGAAC484600.11029441804411216Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA481980.10969810897420798No Hit
AAAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACGTCTGA481420.109570653600488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAGCGTCCGTCCCGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA480750.1094181623497873Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACT476060.10835072359488246Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTATTCCCCATGAACGAGGAATTCCCAGATCGGAAGAGCACACGTCTG474360.10796380549608967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATCAGATCGGAAGAGCACACG470420.10706706590241694No Hit
ATGCATGTCTAAGTACGCACGGCCGAGATCGGAAGAGCACACGTCTGAAC464700.10576520029941998Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGAGATCGGAAGAGCACA457790.10419249202726809No Hit
AAGTGCGGGTCATAAGCTTGCGTTGATTAGATCGGAAGAGCACACGTCTG449270.10225334955567121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCACACGTCTGAACT445320.10135433397318208Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTCCCGACCCCTCCACCCGCGCGTCGAGATCGGAAGAGCACACGTCT443570.10095603593030714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)