FastQCFastQC Report
Wed 5 Jul 2023
SRR6939928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23673033
Total Bases736.3 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGA8488903.585894549295817No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG5659752.390800536627478No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT5381152.273113884477752No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT5266322.2246072144621265No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA3576641.5108499193998504No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA3520341.4870675844535848No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA3327191.4054768563031192No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG2245180.948412482675963No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG2190190.9251835199993172No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG2061460.8708051900236019No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT1871180.7904268118073422No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1799510.760151857178588No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGTTA1752840.7404374420463994No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT1744720.7370073788179149No Hit
ATCGTATAGTGGTTAGTACTCTGCGT1396510.5899159604939511No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA1349990.5702649085987418No Hit
TCCTTAGGTCGCTGGTTCGGTTCCGGCTCGAAGGA1254420.529894078211271No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA1216310.5137955918027065No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA1161520.4906511134420334No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGA1153000.48705208158160385No Hit
TTGGTCTAGGGGTATGATTCTCGCTT1101750.46540297561364447No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGGA1003790.42402255765030195No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT1002280.42338470106470943No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGA991500.41883099643379035No Hit
CCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG971120.41022204463619005No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGG934320.39467693049724556No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA874390.36936120521607857No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG849710.35893584062506906No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG844880.3568955443943326No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGA842160.3557465576971062No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA828800.3501030053901416No Hit
TCCTTAGGTCGCTGGTTCGGTTCCGGCTCGAAGG812710.34330624217015204No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA797940.33706707543558106No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG796750.33656439375554453No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG793030.3349929854784556No Hit
CCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGA752990.3180792254207562No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG749790.31672747636519577No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGA719230.30381827288459406No Hit
TTCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG695180.29365903388889797No Hit
CAAACGATGCCGACCGGCGATGCGGCGGCGTTA668420.2823550324117742No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT638240.26960634913152026No Hit
TTCCCATGGTGTAATGGTTAGCACTCTGGA637110.26912901274627543No Hit
ATCGTATAGTGGTTAGTACTCTGCG627770.2651835951903586No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGG608320.25696749546203057No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC558060.23573658685813514No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC543220.22946785061297384No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC528700.22333428927336857No Hit
TTTGGTCTAGGGGTATGATTCTCGCTT525510.2219867644336068No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG509270.21512663797663784No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGT472810.1997251471748466No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG455270.1923158726640562No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG445440.18816346853400662No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTAT434280.18344924370273974No Hit
CGCGGCCCGGGTTCGTTTCCCGGTCAGGGA392870.16595676608062854No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA374880.15835740185889996No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGG368160.15551872884222315No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCG359120.15170003776026503No Hit
CTTAGGTCGCTGGTTCGGTTCCGGCTCGAAGG354820.14988362496685576No Hit
GCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA346290.14628036889062757No Hit
CAAACGATGCCGACCGGCGATGCGGCGGCGTT343740.1452031938619779No Hit
TATAAACGATGCCGACCGGCGATGCGGCGGCGTTA337720.1426602159512049No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG333670.14094940855276128No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG331480.14002430529286214No Hit
CAACGATGCCGACCGGCGATGCGGCGGCGTTA312010.13179975713293687No Hit
TCGCCGCGGCCCGGGTTCGGTTCCCGGTCAGGGA292810.12368926279957451No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGC290600.12275571110807813No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACGG290470.12270079630269598No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACG287650.12150956744748338No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGG281600.11895391688931452No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGA281270.11881451776795986No Hit
CTTAGGTCGCTGGTTCGATTCCGGCTCGAAGG279720.11815976431917279No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG278320.11756837410736513No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG273510.1155365263082259No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGG264160.11158688453651038No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGG263380.11125739570421753No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG261450.11044212205508266No Hit
TGGTCTAGGGGTATGATTCTCGCTT257730.10887071377799372No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAG256140.10819906346601214No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGG250910.10598979860333065No Hit
TGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA250900.105985574387532No Hit
TTAACGATGCCGACCGGCGATGCGGCGGCGTTA248760.105081592206626No Hit
GTCGCTGGTTCGTTTCCGGCTCGAAGGA247850.10469718856895101No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGG241640.10207395055800413No Hit
TTGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA240960.10178670388369752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)