FastQCFastQC Report
Wed 5 Jul 2023
SRR6939938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519096
Total Bases321.1 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATCACCAGTATTAGAGGC1658411.5765708384066461No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT1173781.1158563435489135No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT751530.7144435225232283No Hit
TCATTATTACCCTCACTGTCAACCCAACA542570.5157952736622995No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC447330.425255174018756No Hit
TCATTATTACCCTCACTGTCAACCCAACAC387250.36813999986310614No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT371050.35273943692499815No Hit
ATCGTATAGTGGTTAGTACTCTGCGT329590.3133254036278403No Hit
TATGCTAAGACTTCACCAGTCAAAGCGA322030.3061384742567232No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG255620.24300567273081258No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCC247960.2357236781563739No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC246790.23461141527751053No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT160600.15267471653457673No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG157150.14939496701997965No Hit
TAGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT156170.14846332802742745No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT151940.1444420699269215No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT147570.1402877205417652No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG144480.13735020575912607No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACCC142530.13549643429435382No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC140480.1335475976262599No Hit
CATGCTAAGACTTCACCAGTCAAAGCGA139430.13254941299138254No Hit
CCTCTAGCATCACCAGTATTAGAGGC135940.1292316373954568No Hit
ACGATTAAAGTCCTACGTGATCTGAGT132530.1259899139621884No Hit
TAAGGCCTACTTCACAAAGCGCCTTCC129260.1228812818135703No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGT126420.12018143003923532No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGA120480.11453455696192905No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC117160.11137839221165013No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC113760.10814617529871387No Hit
TCATTATTACCCTCACTGTCAACCCAAC112940.10736664063147631No Hit
AATGCAGCTCAAAACGCTTAGCCTAGCCACAC107720.10240423701808597No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAG105200.10000859389438028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)