FastQCFastQC Report
Wed 5 Jul 2023
SRR6939940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16373365
Total Bases486.7 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCTAGCATCACCAGTATTAGAGGC2917211.781680186082702No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1215580.7424130592581305No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA1200580.7332518391912719No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA775300.4735129278556974No Hit
TCTGGTCCGATGGTAGTGGGTTATCAGAA755800.46160334176878115No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA755380.4613468276069091No Hit
TATGCTAAGACTTCACCAGTCAAAGCGA683940.417714990168484No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT646250.39469589788049064No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT555680.33938045111679854No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG486570.29717165652875877No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCC480350.29337280394103477No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG392480.23970637678937712No Hit
ATCGTATAGTGGTTAGTACTCTGCGT379340.23168114801080902No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT376620.23001991343868533No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC375020.22904271663155376No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT365960.22350933971117115No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA361380.22071211385075704No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG314210.19190313048050905No Hit
ACCTCTAGCATCACCAGTATTAGAGG312690.19097479351373403No Hit
TCATTATTACCCTCACTGTCAACCCAACA282840.17274396558068547No Hit
AGTTCAACTTTAAATTTGCCCACAGAACC269460.1645721572810476No Hit
CCTCTAGCATCACCAGTATTAGAGGC266890.1630025349095925No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA259660.15858682683736666No Hit
CATGCTAAGACTTCACCAGTCAAAGCGA252680.15432380576625512No Hit
CCTGGTCCGATGGTAGTGGGTTATCAGAA244040.1490469430077446No Hit
TCATTATTACCCTCACTGTCAACCCAACAC238990.14596266558523555No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAA237220.1448816416173462No Hit
ATGCTAAGACTTCACCAGTCAAAGCGA235480.14381894008959062No Hit
ACGATTAAAGTCCTACGTGATCTGAGT226250.1381817360084503No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC221060.13501195386531722No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC211710.12930146002364207No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTT204060.12462923778954417No Hit
AGTTCAACTTTAAATTTGCCCACAGAAC176590.10785199010710383No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG175090.10693586810041796No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAG169090.10327138007367453No Hit
GTTCAACTTTAAATTTGCCCACAGAACCC168150.10269727694948473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)