FastQCFastQC Report
Wed 5 Jul 2023
SRR6939946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11583472
Total Bases359.1 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGATCGTATAGTGGTTAGTACTCTGCGT1951471.6847021342133No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGT908030.7839014071083351No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT683790.5903152353629378No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC666160.5750952736796014No Hit
AATGCAGCTCAAAACGCTTAGCCTAGCCACAC652920.5636651946842881No Hit
AGAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC511550.44162061254173185No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACAC464680.4011577875787156No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAGC389690.3364189942359251No Hit
ATCGTATAGTGGTTAGTACTCTGCGT354300.30586684199694186No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGC353350.3050467079300576No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG329390.28436206346421866No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGA317830.27438232681876384No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA306720.2647910747313068No Hit
TAGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT293050.25298977715835114No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCA289270.24972650687116954No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG285090.2461179169768788No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA281380.24291507762094128No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT233780.2018220443749508No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAG231850.2001558772706491No Hit
AAATGCAGCTCAAAACGCTTAGCCTAGCCACAC230830.1992753122725207No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGT224270.19361207071593042No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGCA221940.19160058400451954No Hit
AACTTGACCAACGGAACAAGTTACCCTAGGGATA215960.18643805587823756No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCC211510.1825963752491481No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCC210060.18134459167337738No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG207270.17893598741379096No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT204800.17680363883989186No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCT204450.17650148418367134No Hit
AGAAATAAGGCCTACTTCACAAAGCGCCTTCCC197620.1706051518922824No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACCC192640.1663059227837733No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT192330.1660383000882637No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGAC189250.16337933911352312No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAA181100.15634345211867393No Hit
AATGCAGCTCAAAACGCTTAGCCTAGCCACACC167270.14440402670287458No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG165840.14316950910745932No Hit
TTGGTCTAGGGGTATGATTCTCGCTT165150.14257383278519603No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGC160810.13882711504806158No Hit
AAATCCCCTTGTAAATTTAACTGTTAGTCCAA160540.1385940243132629No Hit
TGTACATGCTAAGACTTCACCAGTCAAAGCGA159490.13768756034460136No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGA153940.1328962507959617No Hit
TACCCTATGGAGCTTTAATTTATTAATGCAAACAG150900.13027182178193206No Hit
TGAAATAAGGCCTACTTCACAAAGCGCCTTCCC150780.1301682258997993No Hit
TGAAATAAGGCCTACTTCACAAAGCGCCTTCC149940.12944305472487005No Hit
TAGCCTGGTGATAGCTGGTTGTCCAAGATAGAA147600.12742293502328145No Hit
ATGCAGCTCAAAACGCTTAGCCTAGCCACACC141970.12256256155321996No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCC141840.12245033268090948No Hit
AACATCACCTCTAGCATCACCAGTATTAGAGGC137430.11864318401253096No Hit
AGTACATGCTAAGACTTCACCAGTCAAAGCGAACT136070.1174690973483598No Hit
TGCAGCTCAAAACGCTTAGCCTAGCCACAC129090.11144327020430489No Hit
AAAAGGACCTGGCGGTGCTTCATATCCCTCTAGA127490.11006199177586824No Hit
TCAAATAAAGTATAGGCGATAGAAATTGAAACC126930.10957854432591542No Hit
GAGAAATAAGGCCTACTTCACAAAGCGCCTTCCCC125030.10793827619214688No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG124710.10766202050645954No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACC117690.10160166140169372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)