FastQCFastQC Report
Wed 5 Jul 2023
SRR6939948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6939948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18779579
Total Bases577.2 Mbp
Sequences flagged as poor quality0
Sequence length25-35
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGATCGTATAGTGGTTAGTACTCTGCGT12079756.432385944328145No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGT10220165.44216672801877No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCG4542572.4188880911547592No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCG3786182.0161154837390125No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCGT3531781.880649188142077No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG3459071.8419316002770882No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG3110921.6565440577768011No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA3065441.632326262479047No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGT2951051.5714143538574532No Hit
TTGGTCTAGGGGTATGATTCTCGCTT2253071.199744680112371No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA2072331.1035018410157118No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGC1944951.0356728444231897No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG1797140.9569650097054891No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1540370.820236704986837No Hit
GTGATCGTATAGTGGTTAGTACTCTGCGT1522540.8107423494424448No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGCG1454490.7745061803568652No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC1272540.6776190243668402No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG1213210.6460261968598976No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGA1146960.6107485157148624No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGG1141110.6076334299080933No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGG1086680.5786498195726326No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1017630.5418811571867506No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG949300.5054958899770863No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG928190.49425495640770223No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA924870.49248707865069813No Hit
ATCGTATAGTGGTTAGTACTCTGCGT913050.4861930078411236No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA911370.48529841909661553No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG876990.46699129943221834No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG873550.465159522479178No Hit
TTTGGTCTAGGGGTATGATTCTCGCTT771910.41103690343644017No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGG737350.39263393497798854No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG671280.3574521026270078No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA661210.3520898950929624No Hit
GCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA651370.3468501610179866No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACGGC650150.34620051919161765No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC646270.34413444518644426No Hit
TCCCATGGTGTAATGGTTAGCACTCTGGA615770.32789339952722046No Hit
GTGATCGTATAGTGGTTAGTACTCTGCG578920.3082710214110764No Hit
CCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA561170.29881926533070846No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGG554640.2953420840797336No Hit
CCCATGGTGTAATGGTTAGCACTCTGGA523880.2789625901624312No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG516130.2748357670850875No Hit
GCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA461210.24559123503247862No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGT457860.24380738247646552No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA456420.24304059212403004No Hit
TCCTTAGGTCGCTGGTTCGGTTCCGGCTCGAAGG436980.23268892236615102No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACGGC427640.22771543494132643No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGT415920.2214746134617821No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGA409850.2182423791289464No Hit
TCGTGATCGTATAGTGGTTAGTACTCTGC409280.217938857947774No Hit
CCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG407490.21698569494023268No Hit
CGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG406540.21647982630494542No Hit
TCCTTAGGTCGCTGGTTCGATTCCGGCTCGAAGG396010.21087267185276093No Hit
CTTAGGTCGCTGGTTCGTTTCCGGCTCGAAG394010.20980768525215607No Hit
TGTGATCGTATAGTGGTTAGTACTCTGCGT388400.2068203978374595No Hit
CGCAGCAACCTCGGTTCGTATCCGAGTCACG383050.20397155868084157No Hit
TGGTCTAGGGGTATGATTCTCGCTT378550.20157533882948067No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA368490.19621845622843836No Hit
TCCCATGGTGTAATGGTTAGCACTCTGG364870.1942908304813436No Hit
CGCAGCAACCTCGGTTCGAATCCGAGTCACG362000.19276257470947566No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG359780.19158043958280427No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGG349100.18589341113557445No Hit
TCGCAGCAACCTCGGTTCGAATCCGAGTCACGG348600.18562716448542324No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCG321680.17129244484128212No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGC319000.16986536279647163No Hit
TTCCCATGGTGTAATGGTTAGCACTCTGGA311460.1658503633121914No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG306500.1632091965426914No Hit
TGATCGTATAGTGGTTAGTACTCTGCGT294550.1568459016040775No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA281870.1500938865562428No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG266000.14164321788044343No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGA262770.13992326452046663No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCG245470.13071113042523477No Hit
TTGATCGTATAGTGGTTAGTACTCTGCGT245290.13061528163118033No Hit
TCGCAGCAACCTCGGTTCGTATCCGAGTCACGG242270.12900715186426703No Hit
GCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG241950.12883675400817027No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACG240000.12779839207258054No Hit
GCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGA232420.1237620928562882No Hit
TTCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGG227900.12135522313892128No Hit
CGCAGCAACCTCGGTTCGGATCCGAGTCACGGC218460.11632848638406645No Hit
CCGTGATCGTATAGTGGTTAGTACTCTG216690.11538597324253115No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG214510.11422513784787187No Hit
ATCGTATAGTGGTTAGTACTCTGCG214120.11401746546075395No Hit
TGCAGCAACCTCGGTTCGAATCCGAGTCACGG209280.11144019788729023No Hit
GTGATCGTATAGTGGTTAGTACTCTGC207510.11049768474575496No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGG207260.11036456142067934No Hit
TCCTTAGGTCGCTGGTTCGGTTCCGGCTCGAAG205930.10965634533127713No Hit
CCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGG197580.10521002627375192No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA196440.10460298391140718No Hit
TCGCCGCGGCCCGGGTTCGGTTCCCGGTCAGGGA187960.10008744072484266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)